Growth of human nonadherent HL-60 cell cultures performed in disposable bioreactor under various hydrodynamic conditions of 2-D wave-assisted agitation has been compared and discussed. Influence of Reynolds number for liquid (Re_L) and the k_La coefficient, as key parameters characterized the bioprocessing of HL-60 cells in ReadyToProcess WAVE^TM 25 system, on reached values of the apparent maximal specific growth rate (μ_max) and the specific yield of biomass (Y^*_X/S) has been identified. The values of Re_L (i.e., 510–10,208), as well as k_La coefficient (i.e., 2.83–13.55 h⁻¹), have been estimated for the cultures subjected to wave-induced mixing, based on simplified dimensionless correlation for various presents of WAVE 25 system. The highest values of apparent μ_max = 0.038 h⁻¹ and Y^*_X/S = 25.64 × 10⁸ cells g_glc⁻¹ have been noted for cultures independently performed at wave-induced agitation characterized by Re_L equaled to 5104 and 510, respectively. The presented results have high applicability potential in scale-up of bioprocesses focused on nonadherent animal cells, or in the case of any application of disposable bioreactors presenting similitude.

已经比较和讨论了在一次性生物反应器中在二维波辅助搅拌的各种流体动力学条件下进行的人类非贴壁 HL-60 细胞培养物的生长。液体雷诺数 (Re _L ) 和k _L a系数的影响，作为表征ReadyToProcess WAVE ^TM 25 系统中 HL-60 细胞生物处理的关键参数，对达到的表观最大比生长率 ( μ _max ) 和已经确定了生物量的比产率 ( Y ^* _X/S )。Re _L的值（即 510–10,208）以及k _L a系数（即，2.83–13.55 h ^-1），已经根据 WAVE 25 系统的各种呈现的简化无量纲相关性，对经受波浪诱导混合的培养物进行了估计。表观μ _max  = 0.038 h ^-1和Y ^* _X/S  = 25.64 × 10 ^{8 个}细胞 g _glc ^-1的最高值已经注意到在以 Re _{L 为}特征的波浪诱导搅拌下独立进行的培养物分别等于 5104 和 510。所呈现的结果在以非粘附动物细胞为重点的生物过程的放大方面具有很高的适用性，或者在任何类似的一次性生物反应器的应用中。