Most of the translational control of gene expression in higher eukaryotes occurs during the initiation step of protein synthesis. While this process is well characterized in mammalian cells, it is less defined in parasites, including the ones that cause human Leishmaniasis. The Leishmania cap-binding isoform 1 (LeishIF4E-1) is the only isoform that binds the specific trypanosomatids-specific hypermethylated 5′ cap, called cap-4, in the human stage of the parasite life cycle. We report here the extensive NMR resonance assignment of LeishIF4E-1 bound to a cap analog, m⁷GTP. The chemical shift data constitute a prerequisite to understanding specific translation initiation mechanisms used in Leishmania parasites and to developing antiparasitic drugs targeting their translation initiation factors.

中文翻译：

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m7GTP 帽结合利什曼原虫起始因子 4E-1 的 1H、13C 和 15N 骨架化学位移分配。

高等真核生物中基因表达的大部分翻译控制发生在蛋白质合成的起始步骤。虽然这一过程在哺乳动物细胞中得到了很好的表征，但它在寄生虫中的定义较少，包括导致人类利什曼病的寄生虫。在利什曼原虫帽结合同种型1（LeishIF4E-1）是结合特定锥虫特异性高甲基化的5'帽的唯一亚型，称为帽4，在寄生虫生命周期的阶段的人类。我们在此报告了与帽类似物 m ⁷ GTP结合的 LeishIF4E-1 的广泛 NMR 共振分配。化学位移数据是理解利什曼原虫中使用的特定翻译起始机制的先决条件 寄生虫和开发针对其翻译起始因子的抗寄生虫药物。