Abstract Phylogenetic species of Paracoccidioides brasiliensis complex (S1a and S1b, PS2, PS3, and PS4) and Paracoccidioides lutzii are agents of paracoccidioidomycosis, an endemic fungal disease in Latin America. P. restrepiensis (PS3 genotype) was classified as monophyletic and geographically restricted to Colombia and neighboring territories. BAT (or Pb-327B) was isolated from a patient living in the southeast region of Brazil but with genotype similar to Colombian Paracoccidioides spp. strains. This study aimed to define the phylogenetic species of BAT isolate by using additional genotyping methods, as well as reviewing the epidemiological and clinical studies related to P. restrepiensis isolates. Genomic DNA of BAT isolate and reference strains of P. brasiliensis sensu stricto (S1b), P. americana (PS2), P. restrepiensis (PS3), and P. lutzii were analyzed by conventional polymerase chain reaction (PCR) of partial gp43 exon 2 loci, by PCR-RFLP technique of tub1 gene, and by sequencing of the whole gp43 exon 2 loci. Here, we show that BAT isolate belongs to P. restrepiensis species, which is an unusual identification in southeastern Brazil, where P. brasiliensis sensu stricto is the prevalent genotype. This identification has relevance for geographical distribution and propagation of the genus Paracoccidioides in South America.

中文翻译：

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副球孢子菌属的表征。来自巴西东南部的菌株，基因型为雷斯特雷氏副球孢子菌 (PS3) 以及该物种系统发育的回顾


摘要 巴西副球孢子菌复合体（S1a 和 S1b、PS2、PS3 和 PS4）和鲁兹副球孢子菌的系统发育物种是副球孢子菌病（拉丁美洲的一种地方性真菌病）的病原体。 P.restrepiensis（PS3 基因型）被归类为单系，地理上仅限于哥伦比亚和邻近地区。 BAT（或 Pb-327B）是从居住在巴西东南部地区的一名患者中分离出来的，但其基因型与哥伦比亚副球孢子菌属相似。菌株。本研究旨在通过使用额外的基因分型方法来定义 BAT 分离株的系统发育物种，并回顾与雷氏假单胞菌分离株相关的流行病学和临床研究。通过部分 gp43 外显子的常规聚合酶链式反应 (PCR) 分析 BAT 分离株和 P. brasiliensis sensu stricto (S1b)、P. americana (PS2)、P.restrepiensis (PS3) 和 P. lutzii 参考菌株的基因组 DNA 2位点，通过tub1基因的PCR-RFLP技术，以及整个gp43外显子2位点的测序。在这里，我们表明 BAT 分离株属于 P.restrepiensis 种，这在巴西东南部是一个不寻常的鉴定，那里 P.brasiliensis sensu stricto 是普遍的基因型。该鉴定与南美洲副球孢子菌属的地理分布和繁殖相关。