Background/objective

The aim was to evaluate the use of bacterial cellulose film and bile duct autograft in repairing critical common bile duct injury in pigs.

Methods

A prospective experimental analytical study was carried out on 20 Sus Domesticus, Piau suidae swine, divided into a control group (n = 10) and an experimental group (n = 10) divided into two subgroups: bacterial cellulose film E1 and bacterial cellulose film E2 to which bacterial cellulose film was randomly allocated. The control group underwent two complete critical common bile duct sections 10 mm apart, while the experimental group with a single critical common bile duct defect underwent a 10 mm section of the longitudinal shaft with edge resection. The defects in the control group were treated with end-to-end conventional anastomosis using polyglycolic 6–0 surgical thread and the experimental group with bacterial cellulose film by continuous suture using the same material. The animals were clinically evaluated throughout the experiment on days D150 (bacterial cellulose film E1), D225 (control group), and D330 (bacterial cellulose film E2) and by intraoperative ultrasound examination related to histopathological and biochemical findings.

Results

The intraoperative ultrasonography detected the changes resulting from the common bile duct anastomosis in the control group that produced a considerable incidence of ductal narrowing and obstruction to the biliary flow. In the bacterial cellulose film E2 group, there was an increase in inflammation intensity, granulomatous reaction, fibrosis, and vessels density, without producing bile duct dilation in the ultrasonography assessment. Biochemical analysis of liver enzymes yielded results in the normal range confirming preservation of liver function at the different post-surgery time points.

Conclusion

Bacterial cellulose film, when used as a graft for bile duct repair, proved to be a biocompatible material that produced a complete healing process and biliary flow continuity.

中文翻译：

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细菌纤维素膜在猪胆管损伤修复中的应用

背景/目标

目的是评估细菌纤维素膜和自体胆管移植物在修复猪严重的胆总管损伤中的应用。

方法

对 20 只 Susdomesticus, Piau suidae 猪进行了前瞻性实验分析研究，分为对照组（n  = 10）和实验组（n = 10) 分为两个亚组：细菌纤维素膜 E1 和细菌纤维素膜 E2，随机分配细菌纤维素膜。对照组接受两个完整的相隔 10 mm 的临界胆总管切片，而具有单个临界胆总管缺损的实验组接受了 10 mm 的纵轴切缘边缘切除术。对照组缺损采用聚乙二醇6-0手术线端对端常规吻合术，实验组采用细菌纤维素膜连续缝合相同材料。在第 150 天（细菌纤维素膜 E1）、第 225 天（对照组）、

结果

术中超声检测到对照组胆总管吻合引起的变化，导致胆管狭窄和胆道阻塞的发生率相当高。在细菌纤维素膜 E2 组中，炎症强度、肉芽肿反应、纤维化和血管密度增加，超声检查未产生胆管扩张。肝酶的生化分析产生的结果在正常范围内，证实了在不同手术后时间点肝功能的保存。

结论

当用作胆管修复移植物时，细菌纤维素膜被证明是一种生物相容性材料，可产生完整的愈合过程和胆汁流动的连续性。