Forty pigs [10.7 ± 1.2 kg initial body weight (BW) at 6 wk of age] were used in a 21-d study to evaluate the effects of supplemental xylanase (Hostazym X 100, Huvepharma, Inc., Peachtree City, GA) in nursery diets on digesta viscosity, nutrient digestibility, health of the small intestine, and growth performance when supplemented with corn distillers’ dried grains with solubles (DDGS). Pigs were individually housed and randomly allotted to four treatments in a 2 × 2 factorial arrangement (n = 20/factor, 0% or 30% DDGS and 0 or 1,500 endo-pentosanase unit/kg xylanase as two factors) based on sex and initial BW. Feed intake and BW were recorded weekly. On day 15 of the study, TiO₂ in diets (0.3%) was used as an indigestible marker to calculate apparent ileal digestibility (AID). Plasma samples were collected on day 19 to measure tumor necrosis factor-alpha (TNF-α), malondialdehyde, and peptide YY. On day 21, all pigs were euthanized to collect tissues from duodenum, jejunum, and colon to measure morphology, TNF-α, and malondialdehyde concentrations. Distal jejunal digesta were collected to measure viscosity. Ileum digesta were collected to measure AID of nutrients. During the entire period, supplemental xylanase increased (P < 0.05) average daily gain (ADG; 616 to 660 g/d) of nursery pigs, whereas DDGS (0 or 30%) did not affect ADG. On week 3, average daily feed intake (ADFI) was increased (P < 0.05) when fed DDGS (1,141 to 1,267 g/d) and there was an interaction (P < 0.05) between two factors indicating that supplemental xylanase decreased ADFI when DDGS was used in a diet. Use of DDGS increased (P < 0.05) viscosity [1.86 to 2.38 centipoise (cP)], whereas supplemental xylanase reduced (P < 0.05) viscosity (2.27 to 1.96 cP) of jejunal digesta. The AID of dry matter (DM) and gross energy (GE) were improved (P < 0.05) by supplemental xylanase. Plasma TNF-α was decreased (P < 0.05, 108.5 to 69.9 pg/mL) by supplemental xylanase. Use of DDGS reduced (P < 0.05) villus height:crypt depth ratio (1.46 to 1.27), whereas supplemental xylanase increased (P < 0.05) the crypt depth (360 to 404 µm) in duodenum. In conclusion, feeding a diet with 30% DDGS to nursery pigs for 3 wk had no negative effect on growth performance, whereas reduced AID of DM and GE, increased TNF-α level in colon tissue, and reduced the ratio of villus height to crypt depth. Dietary supplementation of xylanase reduced digesta viscosity improving AID of nutrients, reduced inflammatory response, and altered intestinal morphology, collectively improving ADG of nursery pigs regardless of the use of DDGS in a diet.

中文翻译：

---

补充木聚糖酶对饲喂玉米酒干粮和可溶物日粮的育苗猪小肠健康的影响。

在21天研究中使用40头猪[ 6周龄时的初始体重（10.7±1.2千克）（BW）]在21天的研究中评估补充木聚糖酶（Hostazym X 100，Huvepharma，Inc.，Peachtree City，GA）的作用。育苗日粮中添加了玉米蒸馏器的干谷粉和可溶物（DDGS）后，其消化粘度，营养物质消化率，小肠的健康状况以及生长性能。根据性别和初始情况，将猪单独饲养，并按2×2因子分解法（n = 20 /因子，0％或30％DDGS和0或1,500戊糖聚糖内切酶单位/ kg木聚糖酶）随机分配给四种处理方法（基于性别和初始值） BW。每周记录采食量和体重。在研究的第15天，TiO ₂日粮中的百分含量（0.3％）被用作计算表观回肠消化率（AID）的难消化指标。在第19天收集血浆样品以测量肿瘤坏死因子-α（TNF-α），丙二醛和肽YY。在第21天，对所有猪实施安乐死以收集十二指肠，空肠和结肠的组织，以测量形态，TNF-α和丙二醛浓度。收集空肠远端消化物以测量粘度。收集回肠回肠以测量营养物的AID。在整个期间，补充木聚糖酶可增加（P <0.05）保育猪的平均日增重（ADG； 616至660 g / d），而DDGS（0或30％）不影响ADG。在第3周，平均每日采食量（ADFI当饲喂DDGS（1,141至1,267 g / d）时）增加（P <0.05），并且两个因素之间存在相互作用（P <0.05），表明当在饮食中使用DDGS时补充木聚糖酶降低了ADFI。DDGS的使用可增加（P <0.05）黏度[1.86至2.38厘泊（cP）]，而补充木聚糖酶可降低空肠消化道的黏度（P <0.05）（2.27至1.96 cP）。补充木聚糖酶可改善干物质（DM）和总能量（GE）的AID （P <0.05）。补充木聚糖酶可降低血浆TNF-α（P <0.05，108.5至69.9 pg / mL）。减少使用DDGS（P<0.05）绒毛高度：隐窝深度比（1.46至1.27），而补充木聚糖酶增加（P <0.05）十二指肠隐窝深度（360至404 µm）。总之，给仔猪饲喂含30％DDGS的饲料3 wk对生长性能没有负面影响，而DM和GE的AID降低，结肠组织中TNF-α水平升高以及绒毛高度与隐窝的比率降低深度。膳食中添加木聚糖酶可降低消化道粘度，从而改善营养成分的AID，降低炎症反应并改变肠道形态，从而共同提高育雏猪的ADG，无论在日粮中使用DDGS如何。