Recent studies have demonstrated that radiation activates in situ antitumor immunity and consequently induced a synergistic effect of radiotherapy and immunotherapy. However, studies related to radiation-induced changes in immune system of tumor-bearing mice are limited, which are of great significance to improve the efficacy of radioimmunotherapy. In this study, we first established a primary lung tumor mouse model using urethane. Then part of the right lung of the mouse was exposed to X-ray irradiation with a computed tomography–guided small animal irradiator and the changes of immune cells in both peripheral blood and spleen were determined by flow cytometry. Besides, the levels of both cytokines and immunoglobulins in mouse serum were detected by a protein chip. We found that B lymphocytes increased while CD8⁺ T lymphocytes reduced significantly. Interleukin-3 (IL-3), IL-6, regulated upon activation, normally T-expressed, and presumably secreted factor (RANTES), and vascular endothelial growth factor (VEGF) were found to be decreased after tumor formation, and the similar results have also been observed with kappa, IgG3, IgE, IgM, and IgG2a. After irradiation, lower concentrations of IgD, kappa, and IgM were found in the serum. Our findings indicate that localized tumor irradiation caused some obvious changes like inhibiting the ability of innate immunity, and these changes may be useful in predicting prognosis.

最近的研究表明，辐射激活原位抗肿瘤免疫力，并因此引起放疗和免疫疗法的协同作用。然而，与辐射诱导的荷瘤小鼠免疫系统变化有关的研究是有限的，对于提高放射免疫疗法的疗效具有重要意义。在这项研究中，我们首先使用氨基甲酸酯建立了原发性肺肿瘤小鼠模型。然后，用计算机断层扫描引导的小动物辐照器对小鼠的右肺部分进行X射线照射，并通过流式细胞仪确定外周血和脾脏中免疫细胞的变化。此外，通过蛋白质芯片检测小鼠血清中细胞因子和免疫球蛋白的水平。我们发现B淋巴细胞增加，而CD8 ⁺T淋巴细胞明显减少。发现白细胞介素3（IL-3），IL-6在激活后受到调节，通常在T表达且可能是分泌因子（RANTES）和血管内皮生长因子（VEGF）降低，且类似。 kappa，IgG3，IgE，IgM和IgG2a也观察到结果。辐照后，血清中发现了较低浓度的IgD，κ和IgM。我们的发现表明，局部肿瘤照射引起了一些明显的变化，例如抑制了先天免疫的能力，这些变化可能有助于预测预后。