Garcinol is a plant-derived compound that has some physiological benefits to human cells. However, the effect of garcinol on ovarian cancer (OC) cell proliferation and apoptosis is unknown. The current study aimed to examine the effects of garcinol alone and in combination with cisplatin (DDP) on cellular behavior and to explore the expression pattern of PI3K/AKT and nuclear factor-κB (NF-κB) in human OC cells. We found that OVCAR-3 cell viability was decreased after garcinol treatment. Garcinol alone and in combination with DDP significantly inhibited cell proliferation and had a synergistic effect evaluated by CompuSyn software. The cell cycle analysis showed the S phase arrest by garcinol. Furthermore, garcinol alone and in combination with DDP promoted cell apoptosis. The garcinol-induced apoptosis was further confirmed by the detection of cleavage forms of PARP and caspase 3. An increase in proapoptotic factor Bax expression was also found in garcinol-treated cells. Moreover, garcinol significantly decreased the phosphorylation of PI3K and AKT proteins and downregulated the expression of NF-κB. Thus, our data demonstrated that garcinol has the potential to be used as an anticancer agent and may synergize the effect of DDP. These actions are most likely through the regulation of the PI3K/AKT and NF-κB pathways.

藤黄酚是一种植物来源的化合物，对人体细胞具有某些生理益处。但是，未知藤黄酚对卵巢癌（OC）细胞增殖和凋亡的影响。当前的研究旨在检查单独的藤黄醇和与顺铂（DDP）联合使用对细胞行为的影响，并探讨PI3K / AKT和核因子-κB（NF-κB）在人OC细胞中的表达模式。我们发现，经糖醇处理后，OVCAR-3细胞的活力降低了。单独使用大蒜素和与DDP联合使用可显着抑制细胞增殖，并通过CompuSyn软件评估具有协同作用。细胞周期分析表明，藤黄将S期阻滞。此外，单独的藤黄酚以及与DDP组合均可促进细胞凋亡。通过检测PARP和caspase 3的切割形式，进一步证实了由garcinol诱导的凋亡。在garcinol处理的细胞中，还发现了促凋亡因子Bax表达的增加。此外，藤黄酚显着降低了PI3K和AKT蛋白的磷酸化，并下调了NF-κB的表达。因此，我们的数据表明，藤黄酚具有用作抗癌药的潜力，并可能协同DDP的作用。这些作用最可能是通过PI3K / AKT和NF-κB途径的调节来实现的。我们的数据表明，藤黄酚具有用作抗癌药的潜力，并可能协同DDP的作用。这些作用最可能是通过PI3K / AKT和NF-κB途径的调节来实现的。我们的数据表明，藤黄酚具有用作抗癌药的潜力，并可能协同DDP的作用。这些作用最可能是通过PI3K / AKT和NF-κB途径的调节来实现的。