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CRISPR-TAPE: protein-centric CRISPR guide design for targeted proteome engineering.
Molecular Systems Biology ( IF 8.5 ) Pub Date : 2020-06-02 , DOI: 10.15252/msb.20209475
Daniel Paolo Anderson 1 , Henry James Benns 1, 2 , Edward William Tate 2 , Matthew Andrew Child 1
Affiliation  

Rational molecular engineering of proteins with CRISPR ‐based approaches is challenged by the gene‐centric nature of gRNA design tools. To address this, we have developed CRISPR ‐TAPE , a protein‐centric gRNA design algorithm that allows users to target specific residues, or amino acid types within proteins. gRNA outputs can be customized to support maximal efficacy of homology‐directed repair for engineering purposes, removing time‐consuming post hoc curation, simplifying gRNA outputs and reducing CPU times.

中文翻译:


CRISPR-TAPE:以蛋白质为中心的 CRISPR 引导设计,用于靶向蛋白质组工程。



使用基于 CRISPR 的方法对蛋白质进行合理的分子工程受到 gRNA 设计工具以基因为中心的性质的挑战。为了解决这个问题,我们开发了 CRISPR ‐TAPE,一种以蛋白质为中心的 gRNA 设计算法,允许用户针对蛋白质内的特定残基或氨基酸类型。 gRNA 输出可以定制,以支持工程目的同源定向修复的最大功效,消除耗时的事后管理,简化 gRNA 输出并减少 CPU 时间。
更新日期:2020-06-30
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