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A two-tier bioinformatic pipeline to develop probes for target capture of nuclear loci with applications in Melastomataceae.
Applications in Plant Sciences ( IF 2.7 ) Pub Date : 2020-05-09 , DOI: 10.1002/aps3.11345
Johanna R Jantzen 1, 2 , Prabha Amarasinghe 1, 2 , Ryan A Folk 3 , Marcelo Reginato 4, 5 , Fabian A Michelangeli 4 , Douglas E Soltis 1, 2 , Nico Cellinese 2 , Pamela S Soltis 2
Affiliation  

Putatively single‐copy nuclear (SCN) loci, which are identified using genomic resources of closely related species, are ideal for phylogenomic inference. However, suitable genomic resources are not available for many clades, including Melastomataceae. We introduce a versatile approach to identify SCN loci for clades with few genomic resources and use it to develop probes for target enrichment in the distantly related Memecylon and Tibouchina (Melastomataceae).

中文翻译:


两层生物信息学管道,用于开发用于核位点目标捕获的探针,并在野牡丹科中应用。



假定单拷贝核(SCN)基因座是使用密切相关物种的基因组资源鉴定的,是系统发育学推断的理想选择。然而,包括野牡丹科在内的许多进化枝都没有合适的基因组资源。我们引入了一种通用方法来识别基因组资源很少的进化枝的 SCN 位点,并用它来开发探针,用于远缘相关的MemecylonTibouchina (Melatomataceae)中的目标富集。
更新日期:2020-05-09
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