Natural biopolymers have found success in tissue engineering and regenerative medicine applications. Their intrinsic biocompatibility and biological activity make them well suited for biomaterials development. Specifically, keratin-based biomaterials have demonstrated utility in regenerative medicine applications including bone regeneration, wound healing, and nerve regeneration. However, studies of structure-function relationships in keratin biomaterials have been hindered by the lack of homogeneous preparations of materials extracted and isolated from natural sources such as wool and hair fibers. Here we present a side-by-side comparison of natural and recombinant human hair keratin proteins K31 and K81. When combined, the recombinant proteins (i.e. rhK31 and rhK81) assemble into characteristic intermediate filament-like fibers. Coatings made from natural and recombinant dimers were compared side-by-side and investigated for coating characteristics and cell adhesion. In compar...

中文翻译：

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由重组 K31 和 K81 组装的材料与提取的人发角蛋白的比较研究。

天然生物聚合物已在组织工程和再生医学应用中取得成功。它们固有的生物相容性和生物活性使它们非常适合生物材料的开发。具体而言，基于角蛋白的生物材料已证明可用于再生医学应用，包括骨再生、伤口愈合和神经再生。然而，由于缺乏从羊毛和头发纤维等天然来源中提取和分离的材料的均质制备，角蛋白生物材料的结构-功能关系的研究受到阻碍。在这里，我们展示了天然和重组人头发角蛋白 K31 和 K81 的并排比较。当组合时，重组蛋白（即rhK31 和rhK81）组装成特征性的中间丝状纤维。由天然和重组二聚体制成的涂层并排比较，并研究涂层特性和细胞粘附。相比之下...