Collective cell migration refers to the movement of groups of cells and collective cell behavior and relies on cell-cell communication and cell-environment interactions. Collective cell migration plays a fundamental role in many aspects of cell biology and pathology. Current protocols for studying collective cell migration either use destructive methods or are not convenient for liquid handling. Here we present a novel 3D-printed insert-array and a 3D-coculture-array for collective cell migration study in high-throughput. The fabricated insert-array is comprised of 96 cylinder shaped inserts which can be placed in each well of a 96-well plate generating watertight contact with the bottom of each well. The insert-array has high manufacturing tolerance, and the coefficient of variations of the insert diameter and circularity are 0.67% and 0.03%, respectively. Each insert generates a circular cell-free area within the well without cell damage and provides convenient access for both...

中文翻译：

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3D 打印插入阵列和 3D 共培养阵列，用于细胞迁移的高通量筛选和应用，以研究分子和细胞影响。

集体细胞迁移是指细胞群的运动和集体细胞行为，依赖于细胞-细胞通讯和细胞-环境相互作用。集体细胞迁移在细胞生物学和病理学的许多方面起着重要作用。当前用于研究集体细胞迁移的协议要么使用破坏性方法，要么不便于液体处理。在这里，我们提出了一种新颖的 3D 打印插入阵列和 3D 共培养阵列，用于高通量集体细胞迁移研究。制造的插入物阵列由 96 个圆柱形插入物组成，它们可以放置在 96 孔板的每个孔中，与每个孔的底部产生不漏水的接触。刀片阵列制造公差高，刀片直径和圆度的变异系数分别为0.67%和0.03%，分别。每个插入物在孔内产生一个圆形无细胞区域而不会损坏细胞，并为两者提供方便的访问...