Syntheses of many commodities that are produced using microorganisms require cofactors such as ATP and NAD(P)H. Thus, optimization of the flux distribution in central carbon metabolism, which plays a key role in cofactor regeneration, is critical for enhancing the production of the target compounds. Since the intracellular and extracellular conditions change over time in the fermentation process, dynamic control of the metabolic system for maintaining the cellular state appropriately is necessary. Here, we review techniques for detecting the intracellular metabolic state with fluorescent sensors and controlling the flux of central carbon metabolism with optogenetic tools, as well as present a prospect of bio-production processes for fine-tuning the flux distribution.

中文翻译：

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用于中央碳代谢的通量控制技术，可有效产生微生物。

使用微生物生产的许多商品的合成都需要辅助因子，例如ATP和NAD（P）H。因此，在中心碳代谢中通量分布的优化在辅因子再生中起关键作用，对于提高目标化合物的产生至关重要。由于细胞内和细胞外条件在发酵过程中随时间变化，因此需要动态控制代谢系统以适当维持细胞状态。在这里，我们回顾了利用荧光传感器检测细胞内代谢状态并使用光遗传学工具控制中心碳代谢通量的技术，并提出了微调通量分布的生物生产过程的前景。