Pectin, as part of the fruit cell wall, can be degraded by brown rot fungi by coordinating the production, secretion, and action of extracellular enzymes. In this study, pectin utilization by the necrotroph Monilinia laxa 8L was studied by in vitro and in silico approaches. A total of 403 genes encoding carbohydrate-active enzymes (CAZymes) were identified, including 38 coding a predicted pectin-degrading activity. Analyzing the differences between M. laxa 8L exoproteomes in media containing glucose and pectin as sole carbon sources, we identified 107 pectin-specific proteins, among them, 64.48% harbor a classical secretory activity, including 42 CAZymes and six pectin-degrading proteins. Analyzing the gene-expression patterns of some pectinase families revealed their possible sequential action in pectin disassembly. We found, in vitro, an early pectin-dependent induction of MlRGAE1, MlPG1, and three members of the rhamnosidase family (MlαRHA2, MlαRHA3, and MlαRHA6) and late response of MlPG2 and MlPNL3. M. laxa 8L has the ability to use both pectin and byproducts as carbon sources, based on a functional pectinolytic machinery encoded in its genome, subjected to pectin-dependent regulation and appropriate secretion mechanisms of these pectinolytic enzymes. Differences in the secretion and transcription profile of M. laxa 8L provided insights into the different mechanisms that contribute to brown rot development.

果胶是水果细胞壁的一部分，可通过协调细胞外酶的产生，分泌和作用而被褐腐真菌降解。在这项研究中，通过体外和计算机模拟方法研究了坏死性死菌Monilinia laxa 8L对果胶的利用。总共鉴定了403个编码碳水化合物活性酶（CAZymes）的基因，其中38个编码预测的果胶降解活性。分析la.mx之间的区别在含有葡萄糖和果胶作为唯一碳源的培养基中的8L外蛋白质组学中，我们鉴定出107种果胶特异性蛋白，其中64.48％具有经典的分泌活性，其中包括42种CAZymes和6种果胶降解蛋白。分析某些果胶酶家族的基因表达模式，揭示了它们在果胶分解中可能的顺序作用。我们发现，在体外，早期果胶依赖性诱导MlRGAE1，MlPG1，而鼠李糖苷酶家族（三个成员MlαRHA2，MlαRHA3和MlαRHA6）和后期响应MlPG2和MlPNL3。腊肠8L能够根据其基因组中编码的功能性果胶分解机制，使用果胶和副产物作为碳源，并受到果胶依赖性调节和这些果胶分解酶的适当分泌机制的作用。M. laxa 8L分泌和转录谱的差异提供了有助于褐腐发生的不同机制的见解。