Diabetic foot ulcer (DFU) is one of diabetic complications, which is frequently present and tormented in diabetes mellitus. Most multipotent mesenchymal stromal cells (MSCs) are capable of immune evasion, providing an allogeneic, ready-to-use, cell product option for therapeutic applications. The beneficial effect of MSCs for the treatment of a variety of traumatic injuries, such as open wounds, has been extensively explored. In this study, a rat DFU model was used to simulate the pathophysiology of clinical patients and to investigate the localization of human umbilical cord mesenchymal stem cells (hUC-MSCs) after intravenous transplantation and its role in DFU healing, so as to evaluate the potential of hUC-MSCs in the treatment of DFU. The diabetic rat model was established by streptozotocin injection, which was used to create full-thickness foot dorsal skin wounds to mimic DFU by a 6-mm skin biopsy punch and a Westcott scissor. The hUC-MSCs were transplanted through femoral vein, and the ulcer cicatrization situation and the fate of hUC-MSCs were evaluated. Our data suggest that intravenously transplantated hUC-MSCs have the ability to migrate and locate to the wound tissue and are helpful to wound healing in DFU rats, partly by regulating inflammation, trans-differentiation and providing growth factors that promote angiogenesis, cell proliferation and collagen deposition. Herein, we demonstrate that hUC-MSC transplantation is able to accelerate DFU healing in rats and transplantation of exogenous stem cells may be a potential strategy for clinical application in DFUs.

中文翻译：

---

静脉移植人脐带间充质干细胞在大鼠糖尿病足溃疡修复中的作用和作用。

糖尿病足溃疡（DFU）是糖尿病并发症之一，在糖尿病中经常出现并折磨。大多数多能间充质基质细胞（MSC）都具有免疫逃逸的能力，为治疗应用提供了同种异体，即用型细胞产品。已经广泛地探索了MSC在治疗各种创伤如开放性伤口中的有益作用。在这项研究中，大鼠DFU模型用于模拟临床患者的病理生理，并研究静脉移植后人脐带间充质干细胞（hUC-MSC）的定位及其在DFU愈合中的作用，从而评估其潜力。 hUC-MSC在治疗DFU中的作用。链脲佐菌素注射液建立糖尿病大鼠模型，通过6毫米皮肤活检打孔器和Westcott剪刀将其用于创建全厚度足背皮肤伤口，以模仿DFU。将hUC-MSCs通过股静脉移植，评估溃疡愈合情况和hUC-MSCs的命运。我们的数据表明，静脉内移植的hUC-MSC具有迁移和定位至伤口组织的能力，并且有助于DFU大鼠的伤口愈合，部分是通过调节炎症，转分化并提供促进血管生成，细胞增殖和胶原蛋白的生长因子来进行的。沉积。在本文中，我们证明了hUC-MSC移植能够促进大鼠DFU的愈合，外源性干细胞的移植可能是DFU临床应用的潜在策略。