Late blight caused by Phytophthora infestans is a devastating disease affecting potato production worldwide. The quantitative resistance is durable, but the underlying molecular and biochemical mechanisms are poorly understood, limiting its application in breeding. Integrated transcriptomics and metabolomics approach was used for the first time to study the hierarchies of molecular events occurring, following inoculation of resistant and susceptible potato genotypes with P. infestans. RNA sequencing revealed a total of 4216 genes that were differentially expressed in the resistant than in the susceptible genotype. Genes that were highly expressed and associated with their biosynthetic metabolites that were highly accumulated, through metabolic pathway regulation, were selected. Quantitative real-time PCR was performed to confirm the RNA-seq expression levels. The induced leucine-rich repeat receptor-like kinases (LRR-RLKs) are considered to be involved in pathogen recognition. These receptor genes are considered to trigger downstream oxidative burst, phytohormone signalling-related genes, and transcription factors that regulated the resistance genes to produce resistance related metabolites to suppress the pathogen infection. It was noted that several resistance genes in metabolic pathways related to phenylpropanoids, flavonoids, alkaloids and terpenoid biosynthesis were strongly induced in the resistant genotypes. The pathway specific gene induction provided key insights into the metabolic reprogramming of induced defence responses in resistant genotypes.

中文翻译：

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勘误表：整合的转录组学和代谢组学揭示了马铃薯抗晚疫病抗性基因的诱导。

由疫霉疫霉引起的晚疫病是一种破坏性疾病，影响全世界的马铃薯生产。定量抗性是持久的，但对潜在的分子和生化机制了解甚少，从而限制了其在育种中的应用。首次使用整合的转录组学和代谢组学方法研究了用致病疫霉接种抗性和易感马铃薯基因型后发生的分子事件的层次。RNA测序显示共有4216个基因在抗性中比在易感基因型中差异表达。通过代谢途径调节，选择高表达的基因及其与高积累的生物合成代谢产物相关的基因。进行定量实时PCR以确认RNA-seq表达水平。诱导的富亮氨酸重复受体样激酶（LRR-RLKs）被认为与病原体识别有关。这些受体基因被认为可以触发下游的氧化爆发，植物激素信号相关的基因以及调节抗性基因产生抗性相关代谢产物以抑制病原体感染的转录因子。注意到代谢途径中的几个抗性基因与苯丙烷有关，在抗性基因型中强烈诱导类黄酮，生物碱和萜类生物合成。途径特异性基因诱导提供了对抗性基因型中诱导的防御反应的代谢重编程的关键见解。