Abstract Fusarium oxysporum f. sp. phaseoli (Fop) J.B. Kendrich & W.C. Snyder is the causal agent of Fusarium wilt of common bean (Phaseolus vulgaris L.). The objective of this study was to develop microsatellite markers (SSRs) to characterize the genetic diversity of Fop. Two libraries enriched with SSRs were developed and a total of 40 pairs of SSRs were characterized. Out of these, 15 SSRs were polymorphic for 42 Fop isolates. The number of alleles varied from two to ten, with an average of four alleles per locus and an average PIC (Polymorphic Information Content) of 0.38. The genetic diversity assessed by microsatellites for Fop was low, as expected for an asexual fungus, and not associated with geographic origin, but they were able to detect enough genetic variability among isolates in order to differentiate them. Microsatellites are a robust tool widely used for genetic fingerprinting and population structure analyses. SSRs for Fop may be an efficient tool for a better understanding of the ecology, epidemiology and evolution of this pathogen.

中文翻译：

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尖孢镰刀菌 f. sp。新开发的微卫星评估菜豆遗传变异性

摘要尖孢镰刀菌 f。sp。菜豆 (Fop) JB Kendrich & WC Snyder 是菜豆 (Phaseolus vulgaris L.) 枯萎病的病原体。本研究的目的是开发微卫星标记（SSR）来表征 Fop 的遗传多样性。开发了两个富含 SSR 的文库，并对总共 40 对 SSR 进行了表征。其中，15 个 SSR 对 42 个 Fop 分离株具有多态性。等位基因的数量从 2 个到 10 个不等，每个位点平均有 4 个等位基因，平均 PIC（多态信息含量）为 0.38。微卫星评估的 Fop 遗传多样性较低，正如对无性真菌的预期一样，并且与地理起源无关，但他们能够检测分离株之间足够的遗传变异性，以便区分它们。微卫星是一种广泛用于遗传指纹和种群结构分析的强大工具。Fop 的 SSR 可能是更好地了解这种病原体的生态学、流行病学和进化的有效工具。