Abstract The measurement of gene expression can provide important information about gene function and the molecular basis for developmental processes. We analyzed the transcriptomes at three different developmental stages of pepper flower [sporogenous cell division, stage (B1); pollen mother cell meiosis, stage (B2); and open flower (B3)]. In the cDNA libraries for B1, B2, and B3: 82718, 77061, and 91491 unigenes were assembled, respectively. A total of 34,445 unigene sequences and 128 pathways were annotated by KEGG pathway analysis. Several genes associated with nectar biosynthesis and nectary development were identified, and 8,955, 12,182, and 23,667 DEGs were identified in the B2 vs B1, B3 vs B1, and B3 vs. B2 comparisons. DEGs were involved in various metabolic processes, including flower development, nectar biosynthesis, and nectary development. According to the RNA-seq data, all 13 selected DEGs showed similar expression patterns after q-PCR analysis. Sucrose-phosphatase, galactinol-sucrose galactosyltransferase, and sucrose synthase played very important roles in nectar biosynthesis, and CRABS CLAW could potentially be involved in mediating nectary development. A significant number of simple sequence repeat and single nucleotide polymorphism markers were predicted in the Capsicum annuum sequences. The new results provide valuable genetic information about flower development in pepper.

中文翻译：

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辣椒（Capsicum annuum L.）蜜腺发育过程中的花转录组动态


摘要 基因表达的测量可以提供有关基因功能的重要信息和发育过程的分子基础。我们分析了辣椒花三个不同发育阶段的转录组[孢子细胞分裂，阶段（B1）；花粉母细胞减数分裂，阶段（B2）；和开放的花朵(B3)]。在B1、B2和B3的cDNA文库中：分别组装了82718、77061和91491个unigene。通过KEGG通路分析共注释了34,445条unigene序列和128条通路。鉴定了与花蜜生物合成和蜜腺发育相关的几个基因，并在 B2 与 B1、B3 与 B1 以及 B3 与 B2 比较中鉴定了 8,955、12,182 和 23,667 个 DEG。 DEGs参与各种代谢过程，包括花发育、花蜜生物合成和蜜腺发育。根据RNA-seq数据，所有13个选定的DEG在q-PCR分析后都显示出相似的表达模式。蔗糖磷酸酶、半乳糖醇-蔗糖半乳糖基转移酶和蔗糖合酶在花蜜生物合成中发挥着非常重要的作用，CRABS CLAW 可能参与介导蜜腺发育。在辣椒序列中预测有大量的简单序列重复和单核苷酸多态性标记。新结果提供了有关辣椒花发育的宝贵遗传信息。