For improving quality control in the fermented tea production process and advancing the corresponding food labeling with function claims, a rapid and robust hesperidin analysis method using LC-MS/MS with the sample dilution approach was developed by following internationally accepted criteria of the Association of Official Analytical Chemists (AOAC). The linear correlation coefficient (r²) of the regression line was 0.9997 in the concentration range of 0.025 – 2.5 mg/L. The matrix effect evaluated using regression line slope values was negligible. The recovery rate of 100.7% indicated improved trueness. The performance of the newly developed method in determining the hesperidin content of fermented tea samples did not significantly vary from that of a well-established, conventional method. The HorRat values of intra- and inter-laboratory reproducibility studies were both within the acceptable range, indicating sufficient accuracy of the newly developed method according to the AOAC criteria.

为了提高发酵茶生产过程中的质量控制并推进相应食品标签的功能声称，按照官方协会的国际公认标准，开发了一种使用 LC-MS/MS 和样品稀释方法的快速、稳健的橙皮苷分析方法。分析化学家（AOAC）。在0.025 – 2.5 mg/L的浓度范围内，回归线的线性相关系数（r ^{2 ）为0.9997。}使用回归线斜率值评估的矩阵效应可以忽略不计。100.7% 的回收率表明真实性有所提高。新开发的方法在测定发酵茶样品中橙皮苷含量方面的性能与成熟的传统方法没有显着差异。实验室内和实验室间重现性研究的 HorRat 值均在可接受的范围内，表明根据 AOAC 标准，新开发的方法具有足够的准确性。