The aim of this study is to determine the cytotoxic effects of tetrasodium ethylenediaminetetraacetic acid (EDTANa₄) when used alone or when combined with sodium hypochlorite (NaOCl), with and without the addition of cetrimide (CTR). Human pulmonary fibroblast cell line was exposed to the following irrigating solutions: group 1, 2.5% NaOCl; group 2, 10% EDTANa₄; group 3, 20% EDTANa₄; group 4, 2.5% NaOCl/5% EDTANa₄; group 5, 2.5% NaOCl/10% EDTANa₄; group 6, 2.5% NaOCl/5% EDTANa₄/0.2% CTR; group 7, 2.5% NaOCl/10% EDTANa₄/0.2% CTR; group 8, control, cells in Dulbecco’s modified Eagle’s medium. Methyl thiazol tetrazolium assay was used to determine the viability of cells after 1 and 24 h. Viability percentages were analyzed for global comparison using the Welch test followed by the Games-Howell test to determine groups with similar viability, and the Student’s t test was used to compare the two times. The lowest viability was obtained with a 2.5% NaOCl solution at both time periods. The association of NaOCl with EDTANa₄ increased the cellular viability in direct relation with the concentration of the chelating agent. Globally, after 24 h of exposure, cell viability reduced. The solutions of EDTANa₄ showed moderate cytotoxic effects when compared with NaOCl alone.

这项研究的目的是确定乙二胺四乙酸四钠（EDTANa ₄）单独使用或与次氯酸钠（NaOCl）组合使用，并添加和不添加西替米德（CTR）的细胞毒性作用。将人肺成纤维细胞系暴露于以下冲洗液中：第1组，2.5％NaOCl；第2组，10％EDTANa ₄； 第3组，20％EDTANa ₄；第4组，2.5％NaOCl / 5％EDTANa ₄；第5组，2.5％NaOCl / 10％EDTANa ₄；第6组，2.5％NaOCl / 5％EDTANa ₄ /0.2% CTR；第7组，2.5％NaOCl / 10％EDTANa ₄/0.2%点击率；第8组，对照，是Dulbecco改良的Eagle培养基中的细胞。甲基噻唑四唑鎓测定法用于确定1和24小时后的细胞活力。使用Welch检验，然后通过Games-Howell检验分析生存力百分比，以进行整体比较，以确定具有相似生存力的组，并使用Student t检验比较两次。在两个时间段内，使用2.5％NaOCl溶液均获得最低的生存力。NaOCl与EDTANa _4的结合增加了细胞活力，与螯合剂的浓度直接相关。在全球范围内，暴露24小时后，细胞活力降低。与单独的NaOCl相比，EDTANa ₄溶液显示出中等的细胞毒性作用。