Dendritic cells (DCs) are play critical roles in the priming and regulation of immune responses. DCs rapidly process and convey these antigens to prime antigen-specific T cells. Therefore, regulation of DCs functions is important for immunity and immunotherapies. Immune adjuvants for DCs activation are needed to improve the efficacy of vaccines against tumors and many infectious diseases. Therefore, we demonstrate that H. fusiformis extract can regulate DCs maturation and activation. H. fusiformis extract induced costimulatory molecules (CD 80 and CD86), antigen-presenting molecules (major histocompatibility complex (MHC) I and II), CCR7 expression, and interleukin (IL)-12 production in DCs. These effects are associated with upregulation of mitogen-activated protein kinase (MAPK) signaling pathway. In addition, H. fusiformis extract induces costimulatory molecules on splenic DCs and activated CD8⁺ T cells in vivo. Taken together, these findings suggest that H. fusiformis extract may be a potential efficient immune therapeutic compound in DCs-mediated immunotherapies.

Abbreviations

CTL: cytotoxic T lymphocytes; DCs: dendritic cells; ERK: extracellular signal-regulated kinases; IL: interleukini; JNK: c-Jun N-terminal kinase; MAPK: mitogen-activated protein kinase; MHC: major histocompatibility complex

中文翻译：

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羊栖菜提取物可增强体外和体内树突状细胞的成熟。

树突状细胞 (DC) 在免疫反应的启动和调节中发挥着关键作用。 DC 快速处理这些抗原并将其传递给抗原特异性 T 细胞。因此，DCs功能的调节对于免疫和免疫治疗非常重要。需要用于树突状细胞活化的免疫佐剂来提高针对肿瘤和许多传染病的疫苗的功效。因此，我们证明梭形梭菌提取物可以调节 DC 的成熟和激活。 H. fusiformis提取物诱导 DC 中的共刺激分子（CD 80 和 CD86）、抗原呈递分子（主要组织相容性复合体 (MHC) I 和 II）、 CCR7表达和白细胞介素 (IL)-12 产生。这些影响与丝裂原激活蛋白激酶 (MAPK) 信号通路的上调有关。此外， H. fusiformis提取物在体内诱导脾 DC 上的共刺激分子并激活 CD8 ⁺ T 细胞。综上所述，这些发现表明梭形梭菌提取物可能是树突状细胞介导的免疫疗法中潜在的有效免疫治疗化合物。

 缩写

CTL：细胞毒性T淋巴细胞； DC：树突状细胞； ERK：细胞外信号调节激酶； IL：白细胞介素； JNK：c-Jun N 末端激酶； MAPK：丝裂原激活蛋白激酶； MHC：主要组织相容性复合体