Bioprocess and Biosystems Engineering ( IF 3.5 ) Pub Date : 2020-05-30 , DOI: 10.1007/s00449-020-02383-9 Daniela Sallet 1 , Lauren Theisen Fischer 1 , Gustavo Ugalde 2 , Marcus Vinícius Tres 3 , Marcio Antonio Mazutti 1 , Raquel Cristine Kuhn 1
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Abstract
The objective of this work was to concentrate, through a membrane separation process, the fatty acids from oil/solvent mixture. The oil was obtained by ultrasound-assisted extraction from freeze-dried cells of Mortierella isabellina. The concentration of the fatty acids was investigated using flat-sheet polymer membranes of ultrafiltration and nanofiltration. The effects of temperature and pressure were evaluated by the retention of the fatty acids. Oil retentions between 45.23 and 58.20% to ultrafiltration membrane and 43.50 and 56.00% to nanofiltration membrane were observed. The best condition for the ultrafiltration membrane was 4 bar and 40 °C and for nanofiltration membrane was 12 bar and 50 °C. The oil contains a high concentration of oleic acid and palmitic acid that is a desirable property for the biodiesel production. The results showed the applicability of this technology in the solvent recovery step whereas the oil recovered contains a high concentration of fatty acids.
Graphic abstract
中文翻译：
使用聚合物膜分离由伊莎贝氏被孢霉产生的微生物油。
摘要
这项工作的目的是通过膜分离过程从油/溶剂混合物中浓缩脂肪酸。该油是通过超声辅助从伊莎贝拉被孢霉的冻干细胞中提取获得的. 使用超滤和纳滤的平板聚合物膜研究脂肪酸的浓度。通过脂肪酸的保留来评估温度和压力的影响。观察到超滤膜的油截留率为 45.23 至 58.20%，纳滤膜的截油率为 43.50 至 56.00%。超滤膜的最佳条件是 4 bar 和 40 °C，纳滤膜的最佳条件是 12 bar 和 50 °C。该油含有高浓度的油酸和棕榈酸，这是生物柴油生产的理想特性。结果表明该技术适用于溶剂回收步骤，而回收的油含有高浓度的脂肪酸。
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