The aim of this study was to improve flavivirus field monitoring in Brazil using a reliable probe-based RT-qPCR assay. Standard flavivirus strains were employed to evaluate the performance of the assay, and its applicability was evaluated using 235 stored pools of Culicidae samples collected between 1993 and 1997 and in 2016. Flavivirus species were identified by sequencing. Sixteen (6.8%) samples tested positive: Ilheus virus, Iguape virus, and Saint Louis encephalitis virus were identified in historical specimens from 1993-1994, while insect-specific flaviviruses were detected in the samples from 2016. This approach was demonstrated to be accurate for flavivirus detection and characterization, and it can be successfully applied for vector surveillance and for monitoring and discovery of insect specific flaviviruses.

这项研究的目的是使用可靠的基于探针的RT-qPCR分析法改善巴西的黄病毒现场监测。使用标准黄病毒株评估该测定的性能，并使用235个储存的葫芦科库评估其适用性样本于1993年至1997年至2016年间收集。通过测序鉴定黄病毒种。十六个（6.8％）样本检测为阳性：1993-1994年的历史样本中鉴定出伊利乌斯病毒，伊瓜普病毒和圣路易斯脑炎病毒，而2016年的样本中检测到昆虫特异性黄病毒。这种方法被证明是准确的用于黄病毒的检测和表征，并且可以成功地用于载体监测以及昆虫特异性黄病毒的监测和发现。