Remorins (REMs) play an important role in the ability of plants to adapt to adverse environments. PeREM6.5, a protein of the REM family in Populus euphratica (salt-resistant poplar), was induced by NaCl stress in callus, roots and leaves. We cloned the full-length PeREM6.5 from P. euphratica and transformed it into Escherichia coli and Arabidopsis thaliana. PeREM6.5 recombinant protein significantly increased the H+-ATPase hydrolytic activity and H+ transport activity in P. euphratica plasma membrane (PM) vesicles. Yeast two-hybrid assay showed that P. euphratica REM6.5 interacted with RPM1-interacting protein 4 (PeRIN4). Notably, the PeREM6.5-induced increase in PM H+-ATPase activity was enhanced by PeRIN4 recombinant protein. Overexpression of PeREM6.5 in Arabidopsis significantly improved salt tolerance in transgenic plants in terms of survival rate, root growth, electrolyte leakage and malondialdehyde content. Arabidopsis plants overexpressing PeREM6.5 retained high PM H+-ATPase activity in both in vivo and in vitro assays. PeREM6.5-transgenic plants had reduced accumulation of Na+ due to the Na+ extrusion promoted by the H+-ATPases. Moreover, the H+ pumps caused hyperpolarization of the PM, which reduced the K+ loss mediated by the depolarization-activated channels in the PM of salinized roots. Therefore, we conclude that PeREM6.5 regulated H+-ATPase activity in the PM, thus enhancing the plant capacity to maintain ionic homeostasis under salinity.

中文翻译：

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胡杨remorin 6.5激活质膜H + -ATPases介导盐耐受性。

Remorins（REM）在植物适应不利环境的能力中起着重要作用。PeREM6.5是胡杨，耐盐杨树中REM家族的一种蛋白，它是由NaCl胁迫诱导的。我们从胡杨假单胞菌中克隆了全长PeREM6.5，并将其转化为大肠杆菌和拟南芥。PeREM6.5重组蛋白显着提高了胡杨假单胞菌质膜（PM）囊泡中的H + -ATPase水解活性和H +转运活性。酵母两杂交试验表明，胡杨假单胞菌REM6.5与RPM1相互作用蛋白4（PeRIN4）相互作用。值得注意的是，PeRIN4重组蛋白增强了PeREM6.5诱导的PM H + -ATPase活性的增加。PeREM6的过表达。在拟南芥中5的存活率，根生长，电解质渗漏和丙二醛含量显着提高了转基因植物的耐盐性。在体内和体外试验中，过表达PeREM6.5的拟南芥植物均保留了较高的PM H + -ATPase活性。由于H + -ATPase促进了Na +的挤出，PeREM6.5转基因植物的Na +积累减少。此外，H +泵引起PM的超极化，从而减少了盐碱化根的PM中由去极化激活通道介导的K +损失。因此，我们得出结论，PeREM6.5调节了PM中的H + -ATPase活性，从而增强了植物在盐度下保持离子稳态的能力。5在体内和体外试验中均保留了较高的PM H + -ATPase活性。由于H + -ATPases促进了Na +的挤出，PeREM6.5转基因植物的Na +积累减少。此外，H +泵引起PM的超极化，从而减少了盐碱化根的PM中由去极化激活通道介导的K +损失。因此，我们得出结论，PeREM6.5调节了PM中的H + -ATPase活性，从而增强了植物在盐度下保持离子稳态的能力。5在体内和体外试验中均保留了较高的PM H + -ATPase活性。由于H + -ATPase促进了Na +的挤出，PeREM6.5转基因植物的Na +积累减少。此外，H +泵引起PM的超极化，从而减少了盐碱化根的PM中由去极化激活通道介导的K +损失。因此，我们得出结论，PeREM6.5调节了PM中的H + -ATPase活性，从而增强了植物在盐度下保持离子稳态的能力。