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Polypyrrole-coated needle as an electrospray emitter for ambient mass spectrometry.
Analytical Methods ( IF 2.7 ) Pub Date : 2020-06-08 , DOI: 10.1039/d0ay00652a Giovanna L de Araújo 1 , Deborah V A de Aguiar 1 , Igor Pereira 1 , Lidya C da Silva 1 , Andrà A R Chaves 1 , Boniek G Vaz 1
Analytical Methods ( IF 2.7 ) Pub Date : 2020-06-08 , DOI: 10.1039/d0ay00652a Giovanna L de Araújo 1 , Deborah V A de Aguiar 1 , Igor Pereira 1 , Lidya C da Silva 1 , Andrà A R Chaves 1 , Boniek G Vaz 1
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Polypyrrole (PPy) is a polymer widely used as an extraction phase due to its ability to perform intermolecular interactions with the analyte, such as acid–base, π–π, dipole–dipole, hydrophobic, and hydrogen bonding. In this manuscript, we report the coating of a stainless steel needle with a PPy film for analyte extraction and subsequent analysis by electrospray ionization mass spectrometry (ESI-MS) under ambient and open-air conditions. The method, named PPy-ESI-MS, was optimized for analysis of 3,4-methylenedioxyamphetamine (MDA) and 3,4-methylenedioxymethamphetamine (MDMA) in synthetic urine. Seven cycles of electrodeposition of the PPy film onto the needle surface, sample at pH 8, and 40 min of extraction of analytes were determined as the best analysis conditions. The analytical performance of PPy-ESI-MS was evaluated for MDA and MDMA compounds. Analytical curves were obtained with R2 > 0.98. Limits of detection (LODs) and limits of quantification (LOQs) were determined as 20 μg L−1 and 70 μg L−1 for MDA and as 25 μg L−1 and 80 μg L−1 for MDMA, respectively. Values of precision were below 17%, and values of accuracy below 5%. The apparent recoveries ranged between 84.5% and 111.3%. In addition, the PPy-ESI-MS method was applied for the analysis of sarcosine in synthetic urine in order to evaluate the performance of the method for another class of compounds. The calibration curve was obtained with R2 > 0.98, along with LOD and LOQ of 30 μg L−1 and 100 μg L−1, respectively. The precision and accuracy values were below 5% and 8%, respectively, and the apparent recoveries close to 100%. This work demonstrates the usefulness of combining an extraction phase with ESI-MS analysis under ambient conditions to determine different classes of small molecules in a complex sample.
中文翻译：
聚吡咯涂层针头用作环境质谱的电喷雾发射器。
聚吡咯（PPy）是一种聚合物，由于其能够与分析物进行分子间相互作用，例如酸碱，π-π，偶极-偶极，疏水键和氢键，因此被广泛用作萃取相。在此手稿中，我们报告了在不锈钢针上涂有PPy膜的涂层，该涂层用于分析物的提取以及随后在环境和露天条件下通过电喷雾电离质谱（ESI-MS）进行的分析。优化了名为PPy-ESI-MS的方法，用于分析合成尿液中的3,4-亚甲二氧基苯丙胺（MDA）和3,4-亚甲二氧基甲基苯丙胺（MDMA）。确定将PPy膜电沉积到针头表面上的七个循环，pH为8的样品以及40分钟的分析物提取作为最佳分析条件。对MDA和MDMA化合物评估了PPy-ESI-MS的分析性能。获得的分析曲线R 2＞ 0.98。检测限（检测限）和定量限（定量限）被确定为20微克大号-1和70微克大号-1为25微克L用MDA和-1和80微克大号-1为MDMA，分别。精度值低于17％，精度值低于5％。表观回收率在84.5％和111.3％之间。此外，将PPy-ESI-MS方法用于合成尿中肌氨酸的分析，以评估该方法对另一类化合物的性能。获得的校准曲线的R 2 > 0.98以及LOD和LOQ为30μgL -1和100μgL -1， 分别。精密度和准确度值分别低于5％和8％，表观回收率接近100％。这项工作证明了在环境条件下将萃取相与ESI-MS分析相结合以测定复杂样品中不同类别的小分子的有用性。
更新日期：2020-07-02
中文翻译：
聚吡咯涂层针头用作环境质谱的电喷雾发射器。
聚吡咯（PPy）是一种聚合物，由于其能够与分析物进行分子间相互作用，例如酸碱，π-π，偶极-偶极，疏水键和氢键，因此被广泛用作萃取相。在此手稿中，我们报告了在不锈钢针上涂有PPy膜的涂层，该涂层用于分析物的提取以及随后在环境和露天条件下通过电喷雾电离质谱（ESI-MS）进行的分析。优化了名为PPy-ESI-MS的方法，用于分析合成尿液中的3,4-亚甲二氧基苯丙胺（MDA）和3,4-亚甲二氧基甲基苯丙胺（MDMA）。确定将PPy膜电沉积到针头表面上的七个循环，pH为8的样品以及40分钟的分析物提取作为最佳分析条件。对MDA和MDMA化合物评估了PPy-ESI-MS的分析性能。获得的分析曲线R 2＞ 0.98。检测限（检测限）和定量限（定量限）被确定为20微克大号-1和70微克大号-1为25微克L用MDA和-1和80微克大号-1为MDMA，分别。精度值低于17％，精度值低于5％。表观回收率在84.5％和111.3％之间。此外，将PPy-ESI-MS方法用于合成尿中肌氨酸的分析，以评估该方法对另一类化合物的性能。获得的校准曲线的R 2 > 0.98以及LOD和LOQ为30μgL -1和100μgL -1， 分别。精密度和准确度值分别低于5％和8％，表观回收率接近100％。这项工作证明了在环境条件下将萃取相与ESI-MS分析相结合以测定复杂样品中不同类别的小分子的有用性。
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