Seed priming is a pre-sown treatment and it is often used to improve the performance of plants in any environment, especially germination. In the current study, various concentrations of nitric oxide (NO) were used to evaluate its role for the induction of physiological variations within seven different wheat (Triticum aestivum L.) genotypes. Two experiments were conducted during 2013 and 2014 and the data were statistically analysed for significance. All these genotypes after treatment with sodium nitroprusside (SNP) as NO donor at 10−4 and 10−5 M concentrations were sown following randomized complete block design with triplicates in the fields of District Muzaffarabad, Pakistan. The concentration of NO at 10−4 M showed promising results and most of the studied characters were found improved compared to control. Wheat varieties primed with 10−4 M SNP showed highest germination speed and germination percentage. NARC-2011 and Uqab-2002 showed much improvement in physiological attributes at both concentrations of NO priming. However, Uqab-2002 and Punjab-2011 showed a significant increase in chlorophyll contents and leaf moisture content with 10−4 and 10−5 M SNP priming compared to control. Highest relative water content was observed within unprimed Lasani, whereas the relative injury was found to be decreased at 10−4 M SNP primed Faisalabad-2008. Wheat varieties Punjab-2011 and Faisalabad-2008 showed the highest increase in grain yield and biological yield by 10−4 M SNP. Hence, it is concluded that sowing of crops after priming at 10−4 M NO concentration can improve the germination, biochemistry and physiology that ultimately lead to an increase in crop yield.

种子引发是一种预先播种的处理方法，通常用于改善任何环境下植物的性能，尤其是发芽。在当前的研究中，使用不同浓度的一氧化氮（NO）评估其在7种不同小麦（Triticum aestivum L.）基因型中诱导生理变异的作用。在2013年和2014年进行了两次实验，并对数据进行统计学分析以确认其重要性。在巴基斯坦穆扎法拉巴德地区进行了三次重复的随机完整区组设计后，播种了以10 -4和10 -5 M浓度的硝普钠（SNP）作为NO供体处理的所有基因型。NO浓度为10 -4M显示出令人鼓舞的结果，并且与对照相比，大多数研究的字符得到改善。用10 -4 M SNP引发的小麦品种发芽速度和发芽率最高。NARC-2011和Uqab-2002在两种NO引发浓度下均显示出生理特性的显着改善。但是，与对照相比，Uqab-2002和Punjab-2011的叶绿素含量和叶片水分含量显着增加，分别以10 -4和10 -5 M SNP引发。在未涂底漆的Lasani中观察到最高相对水含量，而相对损伤在10 -4时降低M SNP启动了Faisalabad-2008。小麦品种Punjab-2011和Faisalabad-2008显示出10 -4 M SNP的最高谷粒产量和生物产量增幅。因此，得出的结论是，以10 -4 M NO浓度引发后播种作物可以改善发芽，生物化学和生理学，最终导致作物产量增加。