ABSTRACT

Objective

To investigate the role of cigarette smoking in disease-development through altering the composition of the oral microbial community. Periodontitis and oral cancer are highly prevalent in Hungary; therefore, the salivary microbiome of smoker and non-smoker Hungarian adults was characterized.

Methods

Shotgun metagenome sequencing of salivary DNA samples from 22 individuals (11 non-smokers and 11 current smokers) was performed using the Ion Torrent PGM^TM platform. Quality-filtered reads were analysed by both alignment-based sequence similarity searches and genome-centric binning.

Results

Prevotella, Veillonella and Streptococcus were the predominant genera in the saliva of both groups. Although the overall composition and diversity of the microbiota were similar, Prevotella was significantly more abundant in salivary samples of current smokers compared to non-smokers. Members of the genus Prevotella were implicated in the development of inflammatory diseases and oral cancer. The abundance of the genus Megasphaera also increased in current smokers, whereas the genera Neisseria, Oribacterium, Capnocytophaga and Porphyromonas were significantly reduced. The data generated by read-based taxonomic classification and genome-centric binning mutually validated the two distinct metagenomic approaches.

Conclusion

Smoking-associated dysbiosis of the salivary microbiome in current cigarette smokers, especially increased abundance of Prevotella and Megasphaera genera, may facilitate disease development.

中文翻译：

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匈牙利吸烟者和非吸烟者唾液微生物组的案例研究：鸟枪宏基因组测序分析。

摘要

客观的

通过改变口腔微生物群落的组成来研究吸烟在疾病发展中的作用。牙周炎和口腔癌在匈牙利非常普遍；因此，对吸烟和不吸烟的匈牙利成年人的唾液微生物组进行了表征。

方法

^{使用 Ion Torrent PGM TM}平台对来自 22 个人（11 名非吸烟者和 11 名当前吸烟者）的唾液 DNA 样本进行鸟枪法宏基因组测序。通过基于比对的序列相似性搜索和以基因组为中心的分箱分析质量过滤的读数。

结果

普氏菌属、韦荣氏菌属和链球菌属是两组唾液中的优势菌属。尽管微生物群的整体组成和多样性相似，但与非吸烟者相比，目前吸烟者的唾液样本中普氏菌的含量明显更高。普氏菌属的成员与炎症性疾病和口腔癌的发展有关。目前吸烟者中巨球菌属的丰度也有所增加，而奈瑟菌属、拟杆菌属、二氧化碳嗜细胞菌属和卟啉单胞菌属显着减少。基于读取的分类学分类和以基因组为中心的分箱生成的数据相互验证了两种不同的宏基因组方法。

结论

当前吸烟者唾液微生物组与吸烟相关的失调，尤其是普氏菌属和巨球菌属的丰度增加，可能会促进疾病的发展。