ABSTRACT The biological control of gastrointestinal nematodes of animals using nematophagous fungi is considered a promising approach. The objective of this study was to evaluate the effectiveness of Arthrobotrys cladodes (CG 719) isolates in vitro and after passage through the gastrointestinal tract of sheep. In experiment 1, the in vitro predatory capacity of A. cladodes against gastrointestinal nematodes in sheep was evaluated. Petri dishes containing fungi cultured on water agar medium were inoculated with 2,000 infective larvae of sheep gastrointestinal nematodes. Experiment 2 involved treatment and control groups comprising six sheep each. The animals in the treatment group received single 10 g doses of pellets containing fungal formulations, and fecal samples were collected after 12, 24, 36, 48, 60, and 72 h. In experiment 1, a larval reduction of 72.3% was observed at 7 days after the larval inoculation. In both assays of experiment 2, larval reductions were significant (p < 0.01). In assay A, this fungus was able to pass throught the gastrointestinal tract of sheep, with a larval reduction of 83.5%. In assay B, proportion of larvae recovered from coprocultures was reduced by 72.4%. Thus, we concluded that pelleted formulations of A. cladodes were effective against gastrointestinal nematodes in vitro and after passage through the gastrointestinal tract of sheep.

中文翻译：

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真菌Arthrobotrys cladodes对绵羊胃肠道线虫的捕食作用

摘要 使用食线虫真菌对动物胃肠道线虫进行生物防治被认为是一种很有前景的方法。本研究的目的是评估Arthrobotrys cladodes (CG 719) 分离株在体外和通过绵羊胃肠道后的有效性。在实验 1 中，评估了 A. cladodes 对绵羊胃肠道线虫的体外捕食能力。含有在水琼脂培养基上培养的真菌的培养皿中接种了 2,000 只感染性绵羊胃肠线虫幼虫。实验 2 涉及治疗组和对照组，每组包括六只羊。治疗组的动物接受单剂 10 g 剂量的含有真菌制剂的颗粒，并在 12、24、36、48、60 和 72 小时后收集粪便样本。在实验 1 中，在幼虫接种后 7 天观察到幼虫减少 72.3%。在实验 2 的两种分析中，幼虫的减少是显着的（p < 0.01）。在测定 A 中，这种真菌能够通过绵羊的胃肠道，幼虫减少了 83.5%。在测定 B 中，从粪培养中回收的幼虫比例降低了 72.4%。因此，我们得出结论，A. cladodes 的颗粒制剂在体外和通过绵羊胃肠道后对胃肠道线虫有效。从粪便中回收的幼虫比例减少了 72.4%。因此，我们得出结论，A. cladodes 的颗粒制剂在体外和通过绵羊胃肠道后对胃肠道线虫有效。从粪便中回收的幼虫比例减少了 72.4%。因此，我们得出结论，A. cladodes 的颗粒制剂在体外和通过绵羊胃肠道后对胃肠道线虫有效。