Semen chilling has some harmful effects on sperm characteristics. Modification of extenders by using conventional and non‐conventional chilling substances can affect sperm characteristics and fertility. One of the important substances using to chilling human blood cells is citric phosphate dextrose (CPD); therefore, it can be used to store ram semen. This study aimed to assay the ability of CPD instead of egg yolk (EGD) and soybean lecithin (SLD)‐based extenders to store ram semen at 5°C. This trial was carried on pooling semen samples that were collected from five rams using a standard artificial vagina and an oestrous restrained ewe. Immediately after semen collection, semen was divided into four portions. The EGD, SLD and citric phosphate dextrose with an antibiotic (CPDA) and without antibiotic (CPD) were extender in the first, second, third and fourth portions of semen, respectively. Sperm characteristics were evaluated after dilution and cooling up to 7 days. Also, conception rate and fertility potential were assayed by 48 ewes (12 each) after 2 days of store ram semen extenders. Data clearly indicated that higher (p > .05) sperm characteristics in EGD, SLD and CPDA than CPD at 0 day of storage. Whilst, sperm characteristics from 1 day up to 7 days of storage were higher (p < .05) in EGD, SLD, CPDA extenders than CPD. Moreover amongst extender types, the advancing of chilling days reduced sperm characteristics. Also, the results showed an acceptable conception rate and the fertility rate of dilution ram semen, which stored 2 days in EGD, SLD and CPDA than CPD. Using of CPD substances with antibiotic in chilling extension ram semen has benefits as well as EGD‐ and SLD‐based extenders. The future work should be conducted to validate these results in frozen ram semen.

中文翻译：

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在冷冻公羊精液中使用柠檬酸磷酸葡萄糖代替蛋黄和大豆卵磷脂来提高生育能力的可能性。

精液冷却对精子特性有一些有害影响。使用常规和非常规冷却物质对稀释剂进行改性会影响精子特性和生育能力。用于冷却人体血细胞的重要物质之一是柠檬酸磷酸葡萄糖 (CPD)；因此，它可用于储存公羊精液。本研究旨在测定 CPD 代替蛋黄 (EGD) 和基于大豆卵磷脂 (SLD) 的增量剂在 5°C 下储存公羊精液的能力。该试验是对使用标准人工阴道和抑制发情的母羊从五只公羊收集的精液样本进行混合进行的。收集精液后立即将精液分成四份。EGD、SLD 和含抗生素 (CPDA) 和不含抗生素 (CPD) 的柠檬酸葡萄糖是第一、第二、分别是精液的第三和第四部分。在稀释和冷却至多 7 天后评估精子特性。此外，在储存公羊精液稀释剂 2 天后，对 48 只母羊（每只 12 只）的受孕率和生育潜力进行了分析。数据清楚地表明，较高（p  > .05) 精子在 EGD、SLD 和 CPDA 中的特征与 CPD 在 0 天储存时相比。同时， 在 EGD、SLD、CPDA 扩展剂中，从储存 1 天到 7 天的精子特征比 CPD更高（p < .05）。此外，在延长剂类型中，寒冷天数的增加降低了精子的特性。此外，结果表明稀释公羊精液在EGD、SLD和CPDA中储存2天的受孕率和受精率均高于CPD。在冷冻延伸公羊精液中使用含有抗生素的 CPD 物质以及基于 EGD 和 SLD 的补充剂都有好处。未来的工作应该在冷冻公羊精液中验证这些结果。