metabolomics is the high‐throughput, multiparametric identification and classification of hundreds of low molecular weight metabolites in a biological sample. Ultimately, metabolites are the downstream readouts of cellular signalling, transcriptomic and proteomic changes that can provide a comprehensive view of tissue and organismal phenotype. The popularity of metabolomics in human sport and exercise has been gaining over the past decade and has provided important insights into the energetic demands and mechanistic underpinnings of exercise and training. To the contrary, metabolomics in the field of equine exercise physiology is lagging despite the horse's superior aerobic and muscular capabilities, as well as its prominence in competitive sport. As such, this narrative review aims to describe metabolomics, its routine implementation, the various analytical methods applied and the state of its use in the equine athlete. Sufficient attention will be paid to methodological considerations, as well as gaps in the equine literature, particularly with regard to the skeletal muscle metabolome. Finally, there will be a brief discussion of the future directions and barriers to metabolomics use in the athletic horse. A thorough understanding of the metabolomics changes that occur in the equine athlete with exercise will undoubtedly help to improve horse management and health across the lifespan.

中文翻译：

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马运动和运动中的代谢组学。

代谢组学是对生物样品中数百种低分子量代谢物的高通量、多参数鉴定和分类。最终，代谢物是细胞信号、转录组和蛋白质组变化的下游读数，可以提供组织和生物体表型的全面视图。在过去的十年中，代谢组学在人类运动和锻炼中越来越受欢迎，并为运动和训练的能量需求和机制基础提供了重要的见解。相反，尽管马具有优越的有氧和肌肉能力，以及在竞技运动中的突出地位，但马运动生理学领域的代谢组学却滞后。因此，这篇叙述性评论旨在描述代谢组学、其常规实施、应用的各种分析方法及其在马运动员中的使用状态。将充分注意方法学方面的考虑以及马学文献中的空白，特别是在骨骼肌代谢组方面。最后，将简要讨论代谢组学在运动马中使用的未来方向和障碍。彻底了解马运动员运动时发生的代谢组学变化无疑将有助于改善马匹的整个生命周期管理和健康。将简要讨论代谢组学在运动马中使用的未来方向和障碍。彻底了解马运动员运动时发生的代谢组学变化无疑将有助于改善马匹的整个生命周期管理和健康。将简要讨论代谢组学在运动马中使用的未来方向和障碍。彻底了解马运动员运动时发生的代谢组学变化无疑将有助于改善马匹的整个生命周期管理和健康。