Metastasis is the leading cause for mortality in melanoma patients. Here, an unbiased mass spectrometry‐based quantitative proteomic method is utilized to assess differential protein expression in a matched pair of primary/metastatic melanoma cell lines (i.e., WM‐115/WM‐266‐4) derived from the same patient. It is found that TBC1D7 is overexpressed in metastatic over primary melanoma cells, and elevated expression of TBC1D7 promotes the invasion of these melanoma cells in vitro, partly through modulating the activities of secreted matrix metalloproteinases 2 and 9. Additionally, interrogation of publicly available data show that higher mRNA expression of TBC1D7 predicts poorer survival in melanoma patients. Together, the results suggest TBC1D7 as a driver for melanoma cell invasion, which is an important element in melanoma metastasis. The proteomic data generated from this study may also be useful for exploring the roles of other proteins in melanoma metastasis.

中文翻译：

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发现 TBC1D7 作为黑色素瘤细胞侵袭的潜在驱动因素。

转移是黑色素瘤患者死亡的主要原因。在这里，基于无偏质谱的定量蛋白质组学方法用于评估来自同一患者的一对匹配的原发性/转移性黑色素瘤细胞系（即 WM-115/WM-266-4）中的差异蛋白表达。发现 TBC1D7 在转移性黑色素瘤细胞中过度表达，TBC1D7 的表达升高促进了这些黑色素瘤细胞在体外的侵袭，部分通过调节分泌的基质金属蛋白酶 2 和 9 的活性。此外，对公开可用数据的询问显示TBC1D7 的较高 mRNA 表达预示着黑色素瘤患者的生存期较差。总之，结果表明 TBC1D7 是黑色素瘤细胞侵袭的驱动因素，这是黑色素瘤转移的重要因素。