Comparative metabolite profiling of raw milk from subclinical and clinical mastitis cows using 1H‐NMR combined with chemometric analysis,International Journal of Food Science & Technology

当前位置： X-MOL 学术 › Int. J. Food Sci. Tech. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Comparative metabolite profiling of raw milk from subclinical and clinical mastitis cows using 1H‐NMR combined with chemometric analysis
International Journal of Food Science & Technology ( IF 2.6 ) Pub Date : 2020-06-08 , DOI: 10.1111/ijfs.14665
Mayuree Luangwilai ₁ , Kiattisak Duangmal _{1,

2} , Nawanon Chantaprasarn ₃ , Sarn Settachaimongkon _{1,

2,

4}

Affiliation

The objectives of this study were to characterise and compare non‐volatile metabolite profiles of raw milk from healthy, subclinical and clinical mastitis cows using a non‐targeted proton nuclear magnetic resonance (¹H‐NMR) metabolomic approach. A total of 46 metabolites were identified. Multivariate analysis allowed discrimination of milk from healthy and mastitis cows according to their ¹H‐NMR metabolite profiles. Significant increases in acetate, formate, lactate, benzoate, hippurate, β‐hydroxybutyrate, valerate, alanine, histidine, isoleucine, leucine, N‐acetylamino acid, phenylalanine, threonine and valine content were associated with extremely high somatic cell count and clinical mastitis status of the cows. Furthermore, significant rises in hippurate, valerate, N‐acetylglucosamine, histidine, isoleucine and leucine content could be used as potential biomarker for indicating subclinical mastitis infection of the udders. Beside the existing biomarkers for diagnosing mastitis in literatures, we found additional candidate metabolites, such as alanine, valerate and N‐acetylglucosamine, in correlation with mastitis status of Thai crossbred Holstein dairy cows.

中文翻译：

使用1H-NMR和化学计量学分析比较亚临床和临床乳腺炎奶牛的原料乳的代谢物谱

这项研究的目的是使用非靶向质子核磁共振（¹ H-NMR）代谢组学方法来表征和比较健康，亚临床和临床乳腺炎奶牛的原奶的非挥发性代谢物谱。总共鉴定出46种代谢物。多变量分析可根据¹ H-NMR代谢物谱对健康和乳腺炎母牛的牛奶进行区分。乙酸盐，甲酸盐，乳酸盐，苯甲酸盐，马尿酸盐，β-羟基丁酸盐，戊酸盐，丙氨酸，组氨酸，异亮氨酸，亮氨酸，N显着增加‐乙酰氨基酸，苯丙氨酸，苏氨酸和缬氨酸的含量与奶牛的体细胞计数极高和临床乳腺炎状况有关。此外，马尿，戊酸，N-乙酰氨基葡萄糖，组氨酸，异亮氨酸和亮氨酸含量的显着增加可用作表明亚临床乳腺炎感染的潜在生物标志物。除了文献中现有的诊断乳腺炎的生物标志物外，我们还发现了其他候选代谢产物，如丙氨酸，戊酸和N-乙酰氨基葡萄糖，与泰国杂交荷斯坦奶牛的乳腺炎状况有关。

更新日期：2020-06-08

点击分享查看原文

点击收藏

阅读更多本刊最新论文