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Hyperboloid-Drum Microdisk Laser Biosensors for Ultrasensitive Detection of Human IgG.
Small ( IF 13.0 ) Pub Date : 2020-06-08 , DOI: 10.1002/smll.202000239 Zhihe Guo 1 , Yingchun Qin 2 , Peizong Chen 3 , Jinliang Hu 1 , Yi Zhou 1 , Xuyang Zhao 1 , Zhiran Liu 1 , Yiyan Fei 1 , Xiaoshun Jiang 2 , Xiang Wu 1
Small ( IF 13.0 ) Pub Date : 2020-06-08 , DOI: 10.1002/smll.202000239 Zhihe Guo 1 , Yingchun Qin 2 , Peizong Chen 3 , Jinliang Hu 1 , Yi Zhou 1 , Xuyang Zhao 1 , Zhiran Liu 1 , Yiyan Fei 1 , Xiaoshun Jiang 2 , Xiang Wu 1
Affiliation
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Whispering gallery mode (WGM) microresonators have been used as optical sensors in fundamental research and practical applications. The majority of WGM sensors are passive resonators that require complex systems, thereby limiting their practicality. Active resonators enable the remote excitation and collection of WGM‐modulated fluorescence spectra, without requiring complex systems, and can be used as alternatives to passive microresonators. This paper demonstrates an active microresonator, which is a microdisk laser in a hyperboloid‐drum (HD) shape. The HD microdisk lasers are a combination of a rhodamine B‐doped photoresist and a silica microdisk. These HD microdisk lasers can be utilized for the detection of label‐free biomolecules. The biomolecule concentration can be as low as 1 ag mL−1, whereas the theoretical detection limit of the biosensor for human IgG in phosphate buffer saline is 9 ag mL−1 (0.06 aM). Additionally, the biosensors are able to detect biomolecules in an artificial serum, with a theoretical detection limit of 9 ag mL−1 (0.06 aM). These results are approximately four orders of magnitude more sensitive than those for the typical active WGM biosensors. The proposed HD microdisk laser biosensors show enormous detection potential for biomarkers in protein secretions or body fluids.
中文翻译：
用于人IgG超灵敏检测的双曲面鼓微盘激光生物传感器。
在基础研究和实际应用中，耳语画廊模式（WGM）微谐振器已用作光学传感器。大多数WGM传感器是需要复杂系统的无源谐振器，从而限制了它们的实用性。有源谐振器可以实现远程激励和WGM调制荧光光谱的采集，而无需复杂的系统，并且可以用作无源微谐振器的替代品。本文演示了一个有源微谐振器，它是一种双曲面鼓（HD）形状的微盘激光器。HD微盘激光器是罗丹明B掺杂光刻胶和二氧化硅微盘的组合。这些高清微盘激光器可用于检测无标记的生物分子。生物分子浓度可以低至1 ag mL -1，而磷酸盐缓冲盐水中人IgG生物传感器的理论检测限为9 ag mL -1（0.06 a M）。另外，生物传感器能够检测人工血清中的生物分子，理论检测极限为9 g mL -1（0.06 a M）。这些结果比典型的有源WGM生物传感器的灵敏度高出大约四个数量级。提议的高清微盘激光生物传感器在蛋白质分泌物或体液中显示出巨大的生物标志物检测潜力。
更新日期：2020-07-02
中文翻译：
用于人IgG超灵敏检测的双曲面鼓微盘激光生物传感器。
在基础研究和实际应用中，耳语画廊模式（WGM）微谐振器已用作光学传感器。大多数WGM传感器是需要复杂系统的无源谐振器，从而限制了它们的实用性。有源谐振器可以实现远程激励和WGM调制荧光光谱的采集，而无需复杂的系统，并且可以用作无源微谐振器的替代品。本文演示了一个有源微谐振器，它是一种双曲面鼓（HD）形状的微盘激光器。HD微盘激光器是罗丹明B掺杂光刻胶和二氧化硅微盘的组合。这些高清微盘激光器可用于检测无标记的生物分子。生物分子浓度可以低至1 ag mL -1，而磷酸盐缓冲盐水中人IgG生物传感器的理论检测限为9 ag mL -1（0.06 a M）。另外，生物传感器能够检测人工血清中的生物分子，理论检测极限为9 g mL -1（0.06 a M）。这些结果比典型的有源WGM生物传感器的灵敏度高出大约四个数量级。提议的高清微盘激光生物传感器在蛋白质分泌物或体液中显示出巨大的生物标志物检测潜力。
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