Briefly depicted are the publications in CYTOMETRY that received the highest frequency of citations. Among them are seminal papers describing application of metachromatic fluorochrome acridine orange to differentially stain DNA versus RNA or to analyze susceptibility of DNA in situ to denaturation; both features being markers of different sections of the cell cycle including identification of noncycling quiescent cells. The papers reviewing detection of cyclins D1, E, A or B1, each in relation to cell cycle phase, were also among the highly cited ones. The highest citation rates received publications describing development of the TUNEL methodology to detect apoptotic DNA fragmentation, and more recently expression of ϒH2AX to reveal DNA damage. © 2020 International Society for Advancement of Cytometry

中文翻译：

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我与 CYTOMETRY 合作的 40 年。

简要描述了 CYTOMETRY 中引用频率最高的出版物。其中包括描述异染性荧光染料吖啶橙在 DNA 与 RNA 差异染色或分析 DNA 原位变性敏感性方面的应用的开创性论文；这两个特征都是细胞周期不同部分的标记，包括识别非循环静止细胞。审查细胞周期蛋白 D1、E、A 或 B1 检测的论文，每篇都与细胞周期阶段有关，也是被高度引用的论文之一。引用率最高的出版物描述了 TUNEL 方法的发展，以检测凋亡 DNA 片段，以及最近的 ϒH2AX 表达以揭示 DNA 损伤。© 2020 国际细胞计量学促进会