Influence of Protein Modification and Glycosylation in the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin: An Electrochemical Analysis.,ChemPlusChem

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Influence of Protein Modification and Glycosylation in the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin: An Electrochemical Analysis.
ChemPlusChem ( IF 3.0 ) Pub Date : 2020-06-08 , DOI: 10.1002/cplu.202000298
Nasim Izadi _{1,

2} , Hana Černocká ₁ , Mojmír Trefulka ₁ , Veronika Ostatná ₁

Affiliation

To investigate glycans’ influence on the behavior of glycoproteins on charged surfaces, avidin and its nonglycosylated and neutralized version neutravidin were studied by label‐free chronopotentiometric stripping (CPS) analysis and alternating current voltammetry combined with a mercury electrode. Despite neutravidin's and avidin's similar size and structure, their CPS responses differed due to the different amounts of catalytically active free amino groups of lysine and arginine residues. Acetylation of the proteins resulted in the suppression of their CPS responses by almost four times for avidin and by about 50 % for neutravidin, respectively. On the other hand, the presence of glycans in the acetylated avidin induced about 30 % higher chronopotentiometric response compared to the acetylated neutravidin. We suggest that the presence, size and composition of the glycans influenced the CPS signal due to differences in the orientation at a charged surface. The obtained results can be utilized in glycoprotein research.

中文翻译：

蛋白质修饰和糖基化对亲和素和中性亲和素催化氢释放反应的影响：电化学分析。

为了研究聚糖对带电表面糖蛋白行为的影响，通过无标记计时电位溶出（CPS）分析和交流伏安法结合汞电极，研究了抗生物素蛋白及其非糖基化和中和型中性亲和素。尽管中性亲和素和亲和素的大小和结构相似，但是由于赖氨酸和精氨酸残基的催化活性游离氨基数量不同，它们的CPS反应也不同。蛋白质的乙酰化导致抗生物素蛋白的CPS响应分别抑制了近四倍，而中性亲和素的抑制了约50％。另一方面，与乙酰化的中性亲和素相比，乙酰化的抗生物素蛋白中聚糖的存在引起计时电位响应高约30％。我们建议，由于带电表面取向的差异，聚糖的大小和组成影响了CPS信号。所得结果可用于糖蛋白研究。

更新日期：2020-06-24

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