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The Role of Tricellulin in Epithelial Jamming and Unjamming via Segmentation of Tricellular Junctions.
Advanced Science ( IF 14.3 ) Pub Date : 2020-06-08 , DOI: 10.1002/advs.202001213 Sophie Lohmann 1 , Costanza Giampietro 2 , Francesca M Pramotton 1 , Dunja Al-Nuaimi 1 , Alessandro Poli 3 , Paolo Maiuri 3 , Dimos Poulikakos 1 , Aldo Ferrari 1, 2, 4
Advanced Science ( IF 14.3 ) Pub Date : 2020-06-08 , DOI: 10.1002/advs.202001213 Sophie Lohmann 1 , Costanza Giampietro 2 , Francesca M Pramotton 1 , Dunja Al-Nuaimi 1 , Alessandro Poli 3 , Paolo Maiuri 3 , Dimos Poulikakos 1 , Aldo Ferrari 1, 2, 4
Affiliation
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Collective cellular behavior in confluent monolayers supports physiological and pathological processes of epithelial development, regeneration, and carcinogenesis. Here, the attainment of a mature and static tissue configuration or the local reactivation of cell motility involve a dynamic regulation of the junctions established between neighboring cells. Tricellular junctions (tTJs), established at vertexes where three cells meet, are ideally located to control cellular shape and coordinate multicellular movements. However, their function in epithelial tissue dynamic remains poorly defined. To investigate the role of tTJs establishment and maturation in the jamming and unjamming transitions of epithelial monolayers, a semi‐automatic image‐processing pipeline is developed and validated enabling the unbiased and spatially resolved determination of the tTJ maturity state based on the localization of fluorescent reporters. The software resolves the variation of tTJ maturity accompanying collective transitions during tissue maturation, wound healing, and upon the adaptation to osmolarity changes. Altogether, this work establishes junctional maturity at tricellular contacts as a novel biological descriptor of collective responses in epithelial monolayers.
中文翻译：
通过三细胞连接的分割，三纤维素在上皮干扰和解除干扰中的作用。
汇合单层细胞的集体细胞行为支持上皮发育、再生和癌变的生理和病理过程。在这里，成熟和静态组织结构的实现或细胞运动的局部重新激活涉及对相邻细胞之间建立的连接的动态调节。三细胞连接 (tTJ) 建立在三个细胞相遇的顶点，是控制细胞形状和协调多细胞运动的理想位置。然而，它们在上皮组织动态中的功能仍不清楚。为了研究 tTJ 的建立和成熟在上皮单层的干扰和解除干扰转变中的作用，开发并验证了半自动图像处理流程，能够基于荧光报告基因的定位对 tTJ 成熟状态进行无偏且空间分辨的确定。该软件解决了组织成熟、伤口愈合以及适应渗透压变化期间伴随集体转变的 tTJ 成熟度的变化。总而言之，这项工作将三细胞接触处的连接成熟度确立为上皮单层集体反应的新生物学描述符。
更新日期：2020-08-05
中文翻译：
通过三细胞连接的分割，三纤维素在上皮干扰和解除干扰中的作用。
汇合单层细胞的集体细胞行为支持上皮发育、再生和癌变的生理和病理过程。在这里，成熟和静态组织结构的实现或细胞运动的局部重新激活涉及对相邻细胞之间建立的连接的动态调节。三细胞连接 (tTJ) 建立在三个细胞相遇的顶点，是控制细胞形状和协调多细胞运动的理想位置。然而，它们在上皮组织动态中的功能仍不清楚。为了研究 tTJ 的建立和成熟在上皮单层的干扰和解除干扰转变中的作用，开发并验证了半自动图像处理流程，能够基于荧光报告基因的定位对 tTJ 成熟状态进行无偏且空间分辨的确定。该软件解决了组织成熟、伤口愈合以及适应渗透压变化期间伴随集体转变的 tTJ 成熟度的变化。总而言之，这项工作将三细胞接触处的连接成熟度确立为上皮单层集体反应的新生物学描述符。
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