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Alkaloid chemophenetics and transcriptomics of the Nicotiana genus
Phytochemistry ( IF 3.2 ) Pub Date : 2020-09-01 , DOI: 10.1016/j.phytochem.2020.112424 Kacper Piotr Kaminski 1 , Lucien Bovet 1 , Helene Laparra 1 , Gerhard Lang 1 , Damien De Palo 1 , Nicolas Sierro 1 , Simon Goepfert 1 , Nikolai V Ivanov 1
Phytochemistry ( IF 3.2 ) Pub Date : 2020-09-01 , DOI: 10.1016/j.phytochem.2020.112424 Kacper Piotr Kaminski 1 , Lucien Bovet 1 , Helene Laparra 1 , Gerhard Lang 1 , Damien De Palo 1 , Nicolas Sierro 1 , Simon Goepfert 1 , Nikolai V Ivanov 1
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In this study, we determined the pyridine alkaloid content (nicotine, nornicotine, anabasine, anatabine, cotinine, and myosmine) of 58 species and 2 subspecies of the Nicotiana genus by ultra-high-performance liquid chromatography coupled with mass spectrometry. We observed clear correlation between Noctiflorae and Suaveolentes sections and their above average accumulation of anabasine in the genus. In addition, the results demonstrated the presence of not only trace amounts but quantifiable levels of myosmine, an alkaloid previously detected in only minute quantities, in the leaves and roots of 16 species. In this study, analysis of gene expression of 58 species and 2 subspecies from the Nicotiana genus by mRNA sequencing was performed for the first time. Sequencing reads were mapped against annotated genes of a Nicotiana tabacum reference genome and expression values were subsequently calculated. Hierarchical clustering of alkaloid biosynthesis pathway genes and alkaloid content composition revealed patterns clearly segregating Nicotiana sections. Correlation of gene expression with alkaloid accumulation phenotypes was evident, including low putrescine methyltransferase expression for all species in the Suaveolentes section or clear correlation of nicotine demethylase with conversion rates of nicotine to nornicotine in the majority of species. Multiple additional correlations between alkaloid accumulation and gene expression values were identified, which makes this study an important fundament toward future scientific exploration of the Nicotiana genus.
中文翻译：
烟草属生物碱化学表型和转录组学
在本研究中，我们采用超高效液相色谱-质谱联用技术测定了烟草属 58 个物种和 2 个亚种的吡啶生物碱（尼古丁、降烟碱、新烟碱、新烟草碱、可替宁和肌胺）含量。我们观察到夜花科和Suaveolentes 切片之间存在明显的相关性，并且它们在该属中的假木香碱积累量高于平均水平。此外，结果表明，在 16 个物种的叶子和根中不仅存在微量而且可量化水平的肌苷，这是一种以前仅检测到微量的生物碱。本研究首次通过mRNA测序对烟草属58个物种和2个亚种的基因表达进行了分析。将测序读数映射到烟草参考基因组的注释基因，随后计算表达值。生物碱生物合成途径基因和生物碱含量组成的层次聚类揭示了烟草切片清晰分离的模式。基因表达与生物碱积累表型的相关性很明显，包括 Suaveolentes 部分所有物种的低腐胺甲基转移酶表达或大多数物种中尼古丁脱甲基酶与尼古丁到降烟碱的转化率的明显相关性。确定了生物碱积累和基因表达值之间的多种额外相关性，这使得这项研究成为未来烟草属科学探索的重要基础。
更新日期：2020-09-01
中文翻译：
烟草属生物碱化学表型和转录组学
在本研究中，我们采用超高效液相色谱-质谱联用技术测定了烟草属 58 个物种和 2 个亚种的吡啶生物碱（尼古丁、降烟碱、新烟碱、新烟草碱、可替宁和肌胺）含量。我们观察到夜花科和Suaveolentes 切片之间存在明显的相关性，并且它们在该属中的假木香碱积累量高于平均水平。此外，结果表明，在 16 个物种的叶子和根中不仅存在微量而且可量化水平的肌苷，这是一种以前仅检测到微量的生物碱。本研究首次通过mRNA测序对烟草属58个物种和2个亚种的基因表达进行了分析。将测序读数映射到烟草参考基因组的注释基因，随后计算表达值。生物碱生物合成途径基因和生物碱含量组成的层次聚类揭示了烟草切片清晰分离的模式。基因表达与生物碱积累表型的相关性很明显，包括 Suaveolentes 部分所有物种的低腐胺甲基转移酶表达或大多数物种中尼古丁脱甲基酶与尼古丁到降烟碱的转化率的明显相关性。确定了生物碱积累和基因表达值之间的多种额外相关性，这使得这项研究成为未来烟草属科学探索的重要基础。
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