Abstract Non-contact, noninvasive techniques to control the orientation of single living cells are highly valuable for biological research and clinical applications. We experimentally demonstrate a single-beam, single-fiber optical manipulation technique using an anisotropic, four-lobed light field propagated by low-order fiber mode LP21. The laser beam forms a rotationally non-axisymmetric optical multi-trap that may be directed to a spatial location on-demand, capable of cell translation, rotation, and orientation-holding with emitted power as low as 10 mW. We further developed a T-matrix based simulation method that can numerically model and optimize parameters that vary desired laser trap opto-mechanical properties, such as holding torque and capture efficiency. The demonstrated technique is easy to implement for cell micro-manipulation in complex research environments with multi-side occlusion, such as within a microfluidic channel in a lab-on-chip system, and may be used in conjunction with additional units for low-profile three-dimensional rotation and translation, or with other magnetic or electrical manipulation techniques.

中文翻译：

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使用单光束各向异性光阱控制细胞旋转

摘要 控制单个活细胞方向的非接触、非侵入性技术对于生物学研究和临床应用具有很高的价值。我们使用由低阶光纤模式 LP21 传播的各向异性四瓣光场，通过实验证明了单光束、单光纤光学操作技术。激光束形成旋转非轴对称光学多阱，可根据需要定向到空间位置，能够以低至 10 mW 的发射功率进行细胞平移、旋转和方向保持。我们进一步开发了一种基于 T 矩阵的仿真方法，该方法可以对改变所需激光阱光机械特性（例如保持扭矩和捕获效率）的参数进行数值建模和优化。