A novel strategy to discontinuously increase the biomass concentration in a continuous stirred-tank reactor was evaluated to enhance the performance of dark fermentation. Different concentrations of biomass were evaluated at organic loading rates (OLR) ranging from 90 to 160 g lactose/L-d with a hydraulic retention time (HRT) of 6 h. The study revealed that the discontinuous increase of biomass enhanced the hydrogen (H₂) production rates and carboxylic acids concentrations by 19–25% and 8–23%, respectively. In particular, a maximum H₂ production rate of 30.8 L H₂/L-d with carboxylic acids concentration of 20 g/L was reached at an OLR of 138 g lactose/L-d with a biomass concentration of 15 g volatile suspended solids/L. The analysis of microbial communities showed the co-dominance of Clostridium and lactic acid bacteria. Overall, the discontinuous increase of biomass was an effective strategy to improve the performance of suspended-biomass reactors operated at high OLR and low HRT.

评价了在连续搅拌釜反应器中不连续增加生物质浓度的新策略，以增强黑暗发酵的性能。在有机负荷率（OLR）为90至160 g乳糖/ Ld的有机负荷速率下，以6 h的水力停留时间（HRT）评估了不同浓度的生物质。研究表明，生物量的不连续增加分别提高了氢气（H ₂）的生产率和羧酸浓度的19-25％和8-23％。特别是，最大H ₂生产率为30.8 LH ₂在138 g乳糖/ Ld的OLR和15 g挥发性悬浮固体/ L的生物质浓度下达到20 g / L羧酸的/ Ld。微生物群落分析表明，梭菌和乳酸菌是共有的。总体而言，生物量的不连续增加是提高在高OLR和低HRT下运行的悬浮生物质反应堆性能的有效策略。