Background: γ-Aminobutyric acid (GABA) bypasses the TCA cycle via GABA shunt, suggesting a relationship with respiration. However, little is known about its role in seed germination under salt conditions. Results: In this study, exogenous GABA was shown to have almost no influence on mungbean seed germination, except 0.1 mM at 10 h, while it completely alleviated the inhibition of germination by salt treatment. Seed respiration was significantly inhibited by 0.1 and 0.5 mM GABA, but was evidently enhanced under salt treatment, whereas both were promoted by 1 mM GABA alone or with salt treatment. Mitochondrial respiration also showed a similar trend at 0.1 mM GABA. Moreover, proteomic analysis further showed that 43 annotated proteins were affected by exogenous GABA, even 0.1 mM under salt treatment, including complexes of the mitochondrial respiratory chain. Conclusions: Our study provides new evidence that GABA may act as a signal molecule in regulating respiration of mungbean seed germination in response to salt stress. Normal 0 false false false EN-US X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:8.0pt; mso-para-margin-left:0cm; line-height:107%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri",sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;}

中文翻译：

---

外源γ-氨基丁酸对盐分条件下绿豆萌发种子呼吸和蛋白质表达的调节作用

背景：γ-氨基丁酸 (GABA) 通过 GABA 分流绕过 TCA 循环，表明与呼吸有关。然而，人们对其在盐条件下种子萌发中的作用知之甚少。结果：本研究表明，外源GABA对绿豆种子萌发几乎没有影响，除了10小时0.1mM外，完全缓解了盐处理对萌发的抑制作用。种子呼吸被 0.1 和 0.5 mM GABA 显着抑制，但在盐处理下明显增强，而两者都被 1 mM GABA 单独或盐处理促进。在 0.1 mM GABA 时，线粒体呼吸也显示出类似的趋势。此外，蛋白质组学分析进一步表明，43 个注释蛋白受到外源 GABA 的影响，盐处理下甚至 0.1 mM，包括线粒体呼吸链的复合物。结论：我们的研究提供了新的证据，表明 GABA 可能作为信号分子调节绿豆种子萌发响应盐胁迫的呼吸。正常 0 false false false EN-US X-NONE X-NONE /* 样式定义 */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow：是；mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:8.0pt; mso-para-margin-left:0cm; 行高：107%；mso-分页：寡妇孤儿；字体大小：11.0pt; font-family:"Calibri",sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;}