The use of metal complexes to reduce or inhibit virulence factors of Pseudomonas aeruginosa is a promising strategy for the management and control of infections caused by this multidrug-resistant pathogen. The present study aimed to investigate the anti-quorum sensing activity of sub-minimum inhibitory concentrations (sub-MIC) of copper(II) sulfate pentahydrate-curcumin complex (Cu-CUR), iron(III) nitrate nonahydrate -curcumin complex (Fe-CUR), zinc(II) chloride-curcumin complex (Zn-CUR) and free curcumin (free-CUR) against P. aeruginosa PAO1. Metal-CUR complexes were synthesized and characterized by spectroscopic methods. The effect of sub-MIC (1/4 and 1/16 MIC) concentrations of metal-CUR complexes and free-CUR on cell growth, biofilm formation, motility, alginate and pyocyanin production, H₂O₂ susceptibility and expression of lasI and lasR genes in PAO1 was determined. MIC of metal-CUR complexes and free-CUR was determined as 62.5 and 125 µg/ml, respectively. Metal-CUR complexes at concentration of 62.5 µg/ml significantly reduced the cell growth to 1.5%–3.3%. Although we did not measure the anti-QS activity of metal-CUR complexes directly against PAO1, they indicated anti-QS activity in C. violaceum CV026. Copper-CUR complex at the concentration of 1/4 MIC showed the greatest inhibitory effect on swarming and twitching motilities, biofilm formation, alginate and pyocyanin production, sensitivity to H₂O₂ and reduction in the expression levels of lasI and lasR genes (P < 0.001). Considering the biological effects of Cu-CUR complex and its inhibitory activity on virulence factors, it may be used as an effective compound for treatment and control of infections caused by P. aeruginosa.

使用金属配合物减少或抑制铜绿假单胞菌的毒力因子是一种有前景的策略，用于管理和控制由这种多重耐药性病原体引起的感染。本研究旨在研究五价硫酸铜（II）五水合姜黄素复合物（Cu-CUR），硝酸铁（III）九水合-姜黄素复合物（Fe）的最低抑制浓度（sub-MIC）的抗群体感应活性-CUR），氯化锌（II）-姜黄素复合物（Zn-CUR）和针对铜绿假单胞菌的游离姜黄素（free-CUR）PAO1。合成了金属-CUR配合物并通过光谱法对其进行了表征。亚MIC（1/4和1/16 MIC）浓度的金属CUR配合物和游离CUR对细胞生长，生物膜形成，运动性，藻酸盐和绿脓素生成，H ₂ O ₂敏感性以及lasI和lasI表达的影响确定了PAO1中的lasR基因。金属-CUR复合物和游离CUR的MIC分别确定为62.5和125 µg / ml。浓度为62.5 µg / ml的Metal-CUR复合物可将细胞生长显着降低至1.5％–3.3％。尽管我们没有直接测量金属-CUR复合物对PAO1的抗QS活性，但它们表明了紫花衣藻的抗QS活性。CV026。铜-CUR复合物在1/4 MIC的浓度下对群体和抽搐，生物膜形成，藻酸盐和绿脓素生成，对H ₂ O ₂的敏感性以及lasI和lasR基因表达水平的降低具有最大的抑制作用（P <0.001）。考虑到Cu-CUR复合物的生物学效应及其对毒力因子的抑制活性，它可以用作治疗和控制由铜绿假单胞菌引起的感染的有效化合物。