Background: Nano level chiral separation is necessary and demanding in the development of the drug, genomic, proteomic, and other chemical and the environmental sciences. Few drugs exist in human body cells for some days at nano level concentrations, that are out of the jurisdiction of the detection by standard separation techniques. Likewise, the separation and identification of xenobiotics and other environmental contaminants (at nano or low levels) are necessary for our healthiness.

Discussion: This review article reports the state-of-the-art of nano level chiral analyses by nano liquid chromatography (NLC) and nano capillary electrophoresis (NCE). The optimization procedure of the enantiomeric resolution is also deliberated. Also, the efforts are made to describe the chiral resolution mechanisms of in NLC and NCE. Finally, the future perspectives are also emphasized.

Conclusion: This article will be beneficial for chiral chromatographers, academicians, pharmaceutical industries, environmental researchers and Government regulation authorities.

背景：在药物，基因组，蛋白质组学以及其他化学和环境科学的发展中，纳米级手性分离是必要和要求的。人体细胞中几乎没有药物以纳米级浓度存在几天，这超出了标准分离技术所能检测的范围。同样，对于我们的健康而言，异物和其他环境污染物（纳米或低水平）的分离和鉴定也是必要的。

讨论：这篇综述文章报告了通过纳米液相色谱（NLC）和纳米毛细管电泳（NCE）进行的纳米级手性分析的最新技术。还讨论了对映体分辨率的优化程序。此外，还努力描述NLC和NCE中的手性拆分机理。最后，还强调了未来的前景。

结论：本文对手性色谱学家，院士，制药行业，环境研究人员和政府监管机构都是有益的。