In continuous flow biocatalysis, chemical transformations can occur under milder, greener, more scalable, and safer conditions than conventional organic synthesis. However, the method typically involves extensive screening to optimize each enzyme’s immobilization on its solid support material. The task of weighing solids for large numbers of experiments poses a bottleneck for screening enzyme immobilization conditions. For example, screening conditions often require multiple replicates exploring different support chemistries, buffer compositions, and temperatures. Thus, we report 3D-printed labware designed to measure and handle solids in multichannel format and expedite screening of enzyme immobilization conditions. To demonstrate the generality of these advances, alkaline phosphatase, glucose dehydrogenase, and laccase were screened for immobilization efficiency on seven resins. The results illustrate the requirements for optimization of each enzyme’s loading and resin choice for optimal catalytic performance. Here, 3D-printed labware can decrease the requirements for an experimentalist’s time by >95%. The approach to rapid optimization of enzyme immobilization is applicable to any enzyme and many solid support resins. Furthermore, the reported devices deliver precise and accurate aliquots of essentially any granular solid material.

中文翻译：

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用于高通量固定酶的 3D 打印实验室器具。

在连续流生物催化中，化学转化可以在比传统有机合成更温和、更环保、更可扩展和更安全的条件下发生。然而，该方法通常涉及广泛的筛选，以优化每种酶在其固体支持材料上的固定。大量实验中称量固体的任务给筛选酶固定条件带来了瓶颈。例如，筛选条件通常需要多次重复，探索不同的支持化学物质、缓冲液成分和温度。因此，我们报告了 3D 打印实验室器具，旨在以多通道形式测量和处理固体，并加快酶固定条件的筛选。为了证明这些进展的普遍性，对碱性磷酸酶、葡萄糖脱氢酶和漆酶在七种树脂上的固定效率进行了筛选。结果说明了优化每种酶的负载和树脂选择以获得最佳催化性能的要求。在这里，3D 打印的实验室器具可以将实验人员的时间要求减少 95% 以上。快速优化酶固定化的方法适用于任何酶和许多固体支持树脂。此外，所报道的装置可提供基本上任何颗粒状固体材料的精确等分试样。