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Impeded Molecular Reorganization by Polyethylene Glycol Conjugation Revealed by X-ray Reflectivity and Diffraction Measurements.
Langmuir ( IF 3.7 ) Pub Date : 2020-06-05 , DOI: 10.1021/acs.langmuir.0c01202 Pin Zhang 1 , Tiep Pham 1 , Chang Liu 1 , Paola Leon Plata 1 , Joseph Kalkowski 1 , Gang Cheng 1 , Wei Bu 2 , Binhua Lin 2 , Ying Liu 1, 3
Langmuir ( IF 3.7 ) Pub Date : 2020-06-05 , DOI: 10.1021/acs.langmuir.0c01202 Pin Zhang 1 , Tiep Pham 1 , Chang Liu 1 , Paola Leon Plata 1 , Joseph Kalkowski 1 , Gang Cheng 1 , Wei Bu 2 , Binhua Lin 2 , Ying Liu 1, 3
Affiliation
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Polyethylene glycol (PEG) coatings have been widely applied in pharmaceutical and biomedical systems to prevent nonspecific protein absorption, increase vesicle blood circulation time, and sustain drug release. This study systematically investigated the planar interfacial organization of phospholipid monolayers containing various amounts of PEG conjugations before and after enzyme-catalyzed degradation of the lipids using X-ray reflectivity and grazing incidence X-ray diffraction techniques. Results showed that attaching PEG to the headgroup of the lipids up to 15 mol % had limited effects on molecular packing of the lipid monolayers in the condensed phase at the gas–liquid interface and negligible effects on the enzyme adsorption to the interface. After enzyme-catalyzed degradation, equimolar fatty acids and lyso PC were generated. The fatty acids together with the subphase Ca2+ self-assembled into highly organized multilayer domains at the interface. The X-ray measurements unambiguously revealed that the densely packed PEG markedly hindered microphase separation and formation of the palmitic acid–Ca2+ complexes.
中文翻译：
X射线反射率和衍射测量揭示了聚乙二醇共轭阻碍的分子重组。
聚乙二醇（PEG）涂层已广泛应用于药物和生物医学系统中，以防止非特异性蛋白质吸收，增加囊泡血液循环时间并维持药物释放。这项研究使用X射线反射率和掠入射X射线衍射技术系统地研究了在酶催化降解脂质之前和之后含有各种PEG缀合的磷脂单分子层的平面界面组织。结果表明，将PEG附着在脂质头基上至多15 mol％，对在气液界面处凝结相中脂质单分子层的分子堆积的作用有限，而对酶在界面上的吸附作用可忽略不计。经酶催化降解后，生成了等摩尔脂肪酸和溶血PC。2+在界面处自组装成高度组织的多层结构域。X射线测量清楚地表明，紧密堆积的PEG显着阻碍了棕榈酸-Ca 2+复合物的微相分离和形成。
更新日期：2020-07-07
中文翻译：
X射线反射率和衍射测量揭示了聚乙二醇共轭阻碍的分子重组。
聚乙二醇（PEG）涂层已广泛应用于药物和生物医学系统中，以防止非特异性蛋白质吸收，增加囊泡血液循环时间并维持药物释放。这项研究使用X射线反射率和掠入射X射线衍射技术系统地研究了在酶催化降解脂质之前和之后含有各种PEG缀合的磷脂单分子层的平面界面组织。结果表明，将PEG附着在脂质头基上至多15 mol％，对在气液界面处凝结相中脂质单分子层的分子堆积的作用有限，而对酶在界面上的吸附作用可忽略不计。经酶催化降解后，生成了等摩尔脂肪酸和溶血PC。2+在界面处自组装成高度组织的多层结构域。X射线测量清楚地表明，紧密堆积的PEG显着阻碍了棕榈酸-Ca 2+复合物的微相分离和形成。
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