The present study reports profiling of the elevated carbon dioxide (CO₂) concentration responsive global transcriptome in chickpea, along with a combinatorial approach for exploring interlinks between physiological and transcriptional changes, important for the climate change scenario. Various physiological parameters were recorded in two chickpea cultivars (JG 11 and KAK 2) grown in open top chambers under ambient [380 parts per million (ppm)] and two stressed/elevated CO₂ concentrations (550 and 700 ppm), at different stages of plant growth. The elevated CO₂ concentrations altered shoot and root length, nodulation (number of nodules), total chlorophyll content and nitrogen balance index, significantly. RNA-Seq from 12 tissues representing vegetative and reproductive growth stages of both cultivars under ambient and elevated CO₂ concentrations identified 18,644 differentially expressed genes including 9,687 transcription factors (TF). The differential regulations in genes, gene networks and quantitative real-time polymerase chain reaction (qRT-PCR) -derived expression dynamics of stress-responsive TFs were observed in both cultivars studied. A total of 138 pathways, mainly involved in sugar/starch metabolism, chlorophyll and secondary metabolites biosynthesis, deciphered the crosstalk operating behind the responses of chickpea to elevated CO₂ concentration.

中文翻译：

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CO2浓度升高下鹰嘴豆的分子和生理变化。

本研究报告了鹰嘴豆中升高的二氧化碳（CO ₂）浓度响应全球转录组的概况分析，以及探索生理和转录变化之间的相互联系的组合方法，这对气候变化情景很重要。在不同的阶段，在环境[380 ppm（ppm）]和两个压力/升高的CO ₂浓度（550和700 ppm）下于开放顶室中生长的两个鹰嘴豆品种（JG 11和KAK 2）中记录了各种生理参数植物生长 升高的CO ₂浓度显着改变了枝条和根长，根瘤（根瘤数），总叶绿素含量和氮平衡指数。在环境和升高的CO ₂浓度下，来自代表两个品种营养生长和生殖生长阶段的12个组织的RNA-Seq鉴定出18,644个差异表达基因，其中包括9,687个转录因子（TF）。在两个研究品种中均观察到了基因，基因网络和定量实时聚合酶链反应（qRT-PCR）衍生的胁迫反应性TFs表达动态的差异规律。共有138条途径（主要涉及糖/淀粉代谢，叶绿素和次生代谢产物的生物合成）破译了鹰嘴豆对CO ₂升高响应的串扰。 浓度。