In this study, novel antibacterial composite membranes, namely Chitosan‐Heparin‐Minocycline composite membranes, are prepared from chitosan, heparin, minocycline, and CaCl₂ via ion pairing and complexation. These membranes are characterized using Fourier‐transform infrared spectroscopy, scanning electron microscopy, and energy‐dispersive X‐ray spectroscopy. Release kinetics of minocycline from these membranes is evaluated in vitro. Their antibacterial activities are assessed using agar disk diffusion and biofilm assays in vitro. The chemical and physical characterization confirms the successful synthesis of composite membranes. The two types of minocycline‐containing membranes achieve sustained release of minocycline for 17 days and 4 weeks in simulated body fluid, respectively. They demonstrate sustained potent antimicrobial effects against Staphylococcus aureus and Aggregatibacter actinomycetemcomitans in agar disk diffusion assays. Biofilm assays reveal that both minocycline‐containing composite membranes significantly reduce viability of Aggregatibacter actinomycetemcomitans in 7‐day biofilms. These results suggest that these minocycline‐containing composite membranes can be exploited for antibacterial applications in medicine and dentistry.

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壳聚糖-肝素-明环素复合膜的合成及潜在抗菌应用评价

在这项研究中，由壳聚糖，肝素，米诺环素和CaCl ₂制备了新型抗菌复合膜，即壳聚糖-肝素-明环素复合膜。通过离子配对和络合。这些膜通过傅立叶变换红外光谱，扫描电子显微镜和能量色散X射线光谱进行表征。在体外评估了米诺环素从这些膜的释放动力学。使用琼脂圆盘扩散法和体外生物膜测定法评估其抗菌活性。化学和物理特性证实了复合膜的成功合成。两种含米诺环素的膜分别在模拟体液中实现米诺环素的持续释放17天和4周。他们显示出对金黄色葡萄球菌和放线菌放线菌的持续有效抗菌作用在琼脂圆盘扩散测定中。生物膜测定表明，含米诺环素的两种复合膜均会显着降低7天生物膜中集气放线菌的生存能力。这些结果表明，这些含米诺环素的复合膜可用于医学和牙科领域的抗菌应用。