CD200 is an anti‐inflammatory transmembrane glycoprotein in the immunoglobulin superfamily. The interaction of CD200 and its receptor CD200R has shown to inhibit inflammatory response of myeloid cells to foreign materials. The purpose of this study is to create a CD200 immobilized biomaterial surface through polydopamine coating to suppress macrophage cell adhesion and reduce inflammatory cytokine secretion accordingly by macrophages. In this study, tissue‐culture treated polystyrene (TCPS) surface was modified with biotin through polydopamine coating. Purified CD200‐streptavidin fusion protein was then immobilized onto the biotinylated TCPS surface through the high affinity between biotin and streptavidin. Mouse J774A.1 macrophages were seeded on CD200‐immobilized TCPS surface to evaluate the effect of CD200 on preventing macrophage attachment. The effects of CD200‐immobilized TCPS surface on pro‐inflammatory cytokine secretion from J774A.1 macrophages were measured by enzyme‐linked immunosorbent assay. As a result, CD200‐immobilized TCPS surface suppressed macrophage attachment for up to 9 hr. The level of IL‐6 and TNF‐α secreted from J774A.1 macrophages interacted with CD200‐coated TCPS surface was reduced by 36.3% and 32.4%, respectively.

中文翻译：

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阻断巨噬细胞粘附到 CD200 处理的聚苯乙烯培养表面。

CD200 是免疫球蛋白超家族中的抗炎跨膜糖蛋白。CD200 与其受体 CD200R 的相互作用已显示抑制骨髓细胞对外来物质的炎症反应。本研究的目的是通过聚多巴胺涂层创造一个 CD200 固定化生物材料表面，以抑制巨噬细胞粘附并相应地减少巨噬细胞分泌的炎性细胞因子。在这项研究中，组织培养处理的聚苯乙烯 (TCPS) 表面通过聚多巴胺涂层用生物素进行改性。然后通过生物素和链霉亲和素之间的高亲和力将纯化的 CD200-链霉亲和素融合蛋白固定在生物素化的 TCPS 表面上。将小鼠 J774A.1 巨噬细胞接种在 CD200 固定的 TCPS 表面上，以评估 CD200 对防止巨噬细胞附着的影响。CD200 固定的 TCPS 表面对 J774A.1 巨噬细胞促炎细胞因子分泌的影响通过酶联免疫吸附测定进行测量。结果，CD200 固定的 TCPS 表面抑制巨噬细胞附着长达 9 小时。与 CD200 包被的 TCPS 表面相互作用的 J774A.1 巨噬细胞分泌的 IL-6 和 TNF-α 水平分别降低了 36.3% 和 32.4%。