Gentian virus A (GeVA), a novel tombusvirus isolated from Japanese gentian, has shown only a limited ability to infect Japanese gentians under experimental conditions. In this study, temperature was found to affect the efficient multiplication of GeVA in Japanese gentians. GeVA efficiently multiplied in inoculated leaves of gentians at 18 °C but not at 23 °C. This low-temperature (18 °C)-preferred GeVA multiplication was specifically observed in Japanese gentians and Arabidopsis thaliana but not in other experimental plants, including Nicotiana benthamiana. In A. thaliana, visible defense responses, including pathogenesis-related protein 1 expression, were not detected at 23 °C. Furthermore, several A. thaliana mutants, including those defective in RNA silencing, with altered plant immunities did not allow GeVA to multiply to detectable levels at 23 °C. Taken together, these data suggest that unique interaction between GeVA and gentians/A. thaliana, which is independent of RNA silencing, may underlie the low-temperature-preferred multiplication of GeVA.

龙胆病毒 A (GeVA) 是一种从日本龙胆中分离出来的新型 tombusvirus，在实验条件下仅表现出有限的感染日本龙胆的能力。在这项研究中，发现温度会影响日本龙胆中 GeVA 的有效繁殖。 GeVA 在 18 °C 时在接种的龙胆叶中有效繁殖，但在 23 °C 时则不然。这种低温（18°C）偏好的 GeVA 繁殖在日本龙胆和拟南芥中特别观察到，但在其他实验植物（包括本塞姆氏烟草）中没有观察到。在拟南芥中，23°C 时未检测到明显的防御反应，包括发病机制相关蛋白 1 的表达。此外，一些拟南芥突变体，包括那些RNA沉默缺陷的突变体，具有改变的植物免疫力，不允许GeVA在23°C下繁殖到可检测的水平。综上所述，这些数据表明，GeVA 与龙胆/拟南芥之间的独特相互作用（不依赖于 RNA 沉默）可能是 GeVA 低温偏好增殖的基础。