Gene expression analysis is one of the most common and important studies in biology and biomedicine. No matter for traditional blotting analysis or currently commonly used PCR strategy, all need a stable reference gene for normalizing the gene expression. To screen and select housekeeping genes as the most stable reference genes, quantitative real-time PCR (qRT-PCR) was employed to analyze the expression of sixteen commonly used reference genes (IbelF, Ibα-tubulin, IbHIS, IbCOX, IbGAPDH, IbH2B1, IbARF, IbCYC, Ibβ-tubulin, IbACT, IbEFl-a, IbG14, IbPLD, IbRPL2, IbUBQ, IbUBI) in five different tissues under two different temperature stresses in sweet potato. Data analysis by the Delta CT, geNorm, NormFinder, and BestKeeper programs revealed that IbelF is the most stable gene and IbUBI is the least stable gene as reference. Our study also shows that combination of two or more genes as reference is a better choice, rendering more substantiated expression data for comparison. This study provides evidence for selecting reference genes in sweet potato gene expression analysis.

基因表达分析是生物学和生物医学中最常见，最重要的研究之一。无论是传统的印迹分析还是当前常用的PCR策略，都需要稳定的参考基因来标准化基因表达。为了筛选和选择看家基因作为最稳定的参考基因，采用定量实时PCR（qRT-PCR）分析了16种常用参考基因​​（IbelF，Ibα-微管蛋白，IbHIS，IbCOX，IbGAPDH，IbH2B1，IbARF，IbCYC，Ibβ-微管蛋白，IbACT，IbEFl-a，红薯中两个不同温度胁迫下五个不同组织中的IbG14，IbPLD，IbRPL2，IbUBQ，IbUBI）。通过Delta CT，geNorm，NormFinder和BestKeeper程序进行的数据分析显示，作为参考，IbelF是最稳定的基因，而IbUBI是最不稳定的基因。我们的研究还表明，将两个或多个基因组合作为参考是更好的选择，可以提供更多证实的表达数据进行比较。这项研究为在甘薯基因表达分析中选择参考基因提供了证据。