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Amino acid digestibility and energy use by weaned piglets fed yellow corn, sorghum and an exogenous enzymes combination
Livestock Science ( IF 1.8 ) Pub Date : 2020-06-06 , DOI: 10.1016/j.livsci.2020.104126
Messias Alves Trindade Neto , Connie Gallardo Vela , Julio César Dadalt

In weaned piglet diets, about 20% of the total protein and amino acids (AA) comes from the inclusion of sorghum or yellow corn. Therefore, the fraction of digestible amino acids of these ingredients is important for diet formulations. The ileal-cannulated pig model is not used in younger pigs due to difficulties related to implanting T-cannula in the distal ileum and possible subsequent problems. Thus, the present study evaluated the apparent digestibility of yellow corn and sorghum in piglets by total collection and digestibility of amino acids by the slaughter method. Fifty weaned piglets, at 21-d-old, were divided into two assays to determine effects of multi-carbohydrase (MC) preparation (700 U α-galactosidase, 2,200 U galactomannanase, 3,000 U xylanase, and 22,000 U β-glucanase per kg of diet) and phytase (Phy, 500 FTU per kg of diet) supplementation on the apparent total tract digestibility (ATTD) of dry matter (DM), crude protein (CP), ash, digestible (DE) and metabolizable (ME) energy and standardized ileal digestibility (SID) of AA in yellow corn (YC) and sorghum. Piglets were individually caged and ileal digesta were collected at slaughter (38 d old). A completely randomized experimental design with a 2 × 2 (0 and 200 MC; 0 and 50 Phy) factorial treatment arrangement. A basal diet (BD) was used for an additional group of 5 piglets. Corn BD was used for ATTD determination, and corn-starch BD2 containing 50 g/kg casein was used to estimate AA losses. YC and sorghum replaced 30% of BD. There was no interaction effect (P > 0.05) of the enzyme combination on the apparent digestibility of nutrients, energy, AID and SID of AA of YC and sorghum. The apparent effect from the combination of the enzymes, in fact, was due to the presence of MC, as shown by its isolated inclusion. The supplementation of MC improved ATTD of sorghum DM (P = 0.041) regardless of Phy-supplementation. Piglets weaned at 21 days of age showed lower capacity to use energy and nutrients in the subsequent three weeks, compared to NRC and the Brazilian tables. Isolated or combined, the enzymes did not increase the digestibility coefficients of YC, however, MC increased DM digestibility of sorghum.



中文翻译:

断奶仔猪饲喂黄玉米,高粱和外源酶后的氨基酸消化率和能量消耗

在断奶仔猪日粮中,约20%的总蛋白质和氨基酸(AA)来自高粱或黄玉米。因此,这些成分的可消化氨基酸的比例对于饮食配方很重要。回肠插管的猪模型由于在回肠末端植入T形插管有一定的困难以及可能的后续问题,因此在年幼的猪中不使用回肠插管的猪模型。因此,本研究通过屠宰法通过氨基酸的总收集和消化率评估了仔猪中黄玉米和高粱的表观消化率。五断奶仔猪,在21- d-分为两种测定法,以确定多糖化酶(MC)制剂(每公斤日粮700 Uα-半乳糖苷酶,2,200 U半乳甘露聚糖酶,3,000 U木聚糖酶和22,000 Uβ-葡聚糖酶)和植酸酶(Phy,每千克日粮添加500 FTU)的干物质(DM),粗蛋白(CP),灰分,可消化(DE)和可代谢(ME)能量的表观总道消化率(ATTD)和标准回肠消化率(SID)黄色玉米(YC)和高粱中的AA。将仔猪分别关在笼子里,并在屠宰(38 d大)处收集回肠消化物。完全随机的实验设计,采用2×2(0和200 MC; 0和50 Phy)析因处理方案。基础饮食(BD)用于另外5头仔猪。玉米BD用于ATTD测定,玉米淀粉BD 2含有50克/千克酪蛋白的酪蛋白被用于估计AA损失。YC和高粱取代了BD的30%。酶组合对YC和高粱AA的养分,能量,AID和SID的表观消化率没有交互作用(P > 0.05)。实际上,酶结合产生的明显效果是由于MC的存在,如其分离的包涵体所示。MC的添加改善了高粱DM的ATTD(P  = 0.041),而与Phy的添加无关。与NRC和巴西餐桌相比,在21日龄断奶的仔猪在随后的三周内消耗能量和养分的能力较低。分离或组合后,这些酶没有增加YC的消化率,但是,MC增加了高粱的DM消化率。

更新日期:2020-06-06
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