Chronic hepatitis B (CHB) particularly affects resource-limited countries. CHB management in these areas faces many obstacles for optimal care of patients, including poor access to HBV-DNA quantification, a key marker. This study aims to evaluate the quantification of HBV-DNA on dried blood spots (DBS) using rapid, standardized and fully automated on-demand systems. After a simple and rapid DBS elution protocol, HBV-DNA was simply and accurately quantified on this matrix using two different systems. Limit of quantification was estimated at 400 IU/mL. DBS and plasma HBV-DNA quantification provided comparable results. HBV-DNA stability for up to one month was demonstrated on DBS stored at room temperature, a condition compatible for preservation or transport before analysis. The combined use of DBS and commercially available automated molecular on-demand systems for HBV-DNA quantification could represent a reliable alternative in resource-limited countries to reach remote populations. The good sensitivity of this approach makes it attractive for mother-to-child transmission prevention, treatment decision and follow-up. Costs can be limited if such systems are also validated for other molecular markers.

慢性乙型肝炎（CHB）特别影响资源有限的国家。在这些地区，CHB的管理面临着许多障碍，无法为患者提供最佳护理，包括无法获得HBV-DNA定量指标（关键指标）。这项研究旨在评估使用快速，标准化和全自动的按需系统对干血斑（DBS）上HBV-DNA的定量。经过简单，快速的DBS洗脱方案后，使用两种不同的系统在该基质上简单，准确地定量了HBV-DNA。定量限估计为400 IU / mL。DBS和血浆HBV-DNA定量提供了可比的结果。在室温下保存的DBS上证明了HBV-DNA长达一个月的稳定性，该条件适合分析前保存或运输。DBS和市售的自动分子按需系统结合使用进行HBV-DNA定量分析可能是资源有限的国家向偏远人群提供可靠的替代方法。这种方法的良好敏感性使其对预防母婴传播，治疗决策和随访具有吸引力。如果此类系统也已通过其他分子标记的验证，则可能会限制成本。